RT Journal Article T1 qPCR as a powerful tool for microbial food spoilage quantification: significance for food quality A1 Martínez Álvarez, Noelia A1 Martín, María Cruz A1 Fresno Herrero, Ana A1 Fernández García, María A1 Álvarez González, Miguel Ángel A1 Ladero Losada, Víctor K1 qPCR K1 Food K1 Quality K1 Microorganisms K1 Spoilage K1 Microbial quantification AB The use of real time quantitative PCR (qPCR) has recently been extended to food science. The literature has mainly focused on its use in ensuring food safety. However, it offers a number of advantages with respect to the quantification of non-pathogenic food spoilage microorganisms. Indeed, qPCR may have a promising future in improving the quality of food products. The present review examines the use of qPCR in this area, the basis of the technique, the requirements that must be met for optimal qPCR assays to be performed, and the advantages it offers over other techniques PB Elsevier SN 0924-2244 SN 1879-3053 YR 2011 FD 2011-07 LK https://hdl.handle.net/10347/45528 UL https://hdl.handle.net/10347/45528 LA eng NO Noelia Martínez, Maria Cruz Martín, Ana Herrero, María Fernández, Miguel A. Alvarez, Victor Ladero, qPCR as a powerful tool for microbial food spoilage quantification: Significance for food quality, Trends in Food Science & Technology, Volume 22, Issue 7, 2011, Pages 367-376, ISSN 0924-2244, https://doi.org/10.1016/j.tifs.2011.04.004 NO This work was performed with financial support from the Ministry of Science and Innovation, Spain (AGL2010-18430), and the European Community’s Seventh Framework Programme (KBBE-CT-2007-211441). N. Martínez is the beneficiary of a JAE - CSIC contract financed by the European Social Fund. The authors thank Adrian Burton for linguistic assistance DS Minerva RD 23 may 2026