RT Journal Article T1 Optimizing volatile fatty acids production from fsh canning wastewater: the role of feeding strategies and retention time A1 Casero-Díaz, Tamara A1 Castro‑Barros, Celia A1 Carballa Arcos, Marta A1 Mauricio Iglesias, Miguel A1 Duarte, Maria Salomé Lira K1 Anaerobic fermentation K1 Carboxylate platform K1 Industrial wastewater K1 Resource recovery AB The transition to a circular economy requires innovative strategies for wastewater valorization, particularly in water-intensive sectors, such as fsh canning. Anaerobic fermentation of fsh canning wastewater to produce volatile fatty acids (VFAs) is a promising alternative to reach this goal. This study evaluated the impact of two bioreactor feeding strategies (continuous (UASB) and sequential batch (SBR)) and two hydraulic retention times (HRT) (6 and 3 days) on VFA yield and product spectrum. The results showed that both feeding modes resulted in high VFA production, exceeding 30 g VFA-COD/L. It can be concluded that the feeding mode has no impact on VFA yield. However, it does exert a signifcant infuence on the product spectrum. Sequential feeding was found to favor butyric acid production, with a 15% higher yield compared to continuous feeding. In contrast, continuous feeding promoted the formation of propionic and valeric acids, with yields 5% and 4% higher, respectively, than those observed under sequential feeding. In contrast, variations in HRT demonstrated no signifcant impact on either yield or product spectrum. These fndings suggest that, for the scaling up of this valorization, the selection of feeding mode should be tailored to the desired objective. Furthermore, a lower HRT (3 days) may lead to financial savings and enhanced productivity without any detrimental impact on yield or product spectrum. PB Springer SN 2190-6815 YR 2025 FD 2025-04-08 LK https://hdl.handle.net/10347/42443 UL https://hdl.handle.net/10347/42443 LA eng NO Casero-Díaz, T., Castro-Barros, C., Carballa, M. et al. Optimizing volatile fatty acids production from fish canning wastewater: the role of feeding strategies and retention time. Biomass Conv. Bioref. (2025). https://doi.org/10.1007/s13399-025-06841-w NO Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. This research was funded by Unidad Mixta CIGAT CIRCULAR (IN853 C2022/03) from Axencia Galega de Innovación (GAIN) (Xunta de Galicia). Special thanks to IACOBUS stay program (GNP-AECT) for their support through the grant (104). The authors also thank the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit. MSD acknowledges FCT for the Junior Research contract obtained under the scope of the Scientifc Stimulus Employment 2022 (ref: 2022.06569.CEECIND/CP1718/CT0004; doi:https://doi.org/https://doi.org/10.54499/2022.06569.CEECIND/ CP1718/CT0004). MMI and MC belong to a Galician Competitive Research Group (ED431 C- 2021/37). DS Minerva RD 22 abr 2026