RT Journal Article
T1 Genomic Analysis of Antimicrobial Resistance and Resistance Plasmids in Salmonella Serovars from Poultry in Nigeria
A1 Jibril, Abdurrahman Hassan
A1 Okeke, Iruka N.
A1 Dalsgaard, Anders
A1 García Menéndez, Vanesa
A1 Olsen, John Elmerdahl
K1 Resistance
K1 Plasmids
K1 Salmonella
K1 Poultry
K1 Salmonella genomic islands
K1 Whole genome sequence
K1 Nigeria
AB Antimicrobial resistance is a global public health concern, and resistance genes in Salmonella, especially those located on mobile genetic elements, are part of the problem. This study used phenotypic and genomic methods to identify antimicrobial resistance and resistance genes, as well as the plasmids that bear them, in Salmonella isolates obtained from poultry in Nigeria. Seventy-four isolates were tested for susceptibility to eleven commonly used antimicrobials. Plasmid reconstruction and identification of resistance and virulence genes were performed with a draft genome using in silico approaches in parallel with plasmid extraction. Phenotypic resistance to ciprofloxacin (50.0%), gentamicin (48.6%), nalidixic acid (79.7%), sulphonamides (71.6%) and tetracycline (59.5%) was the most observed. Antibiotic resistance genes (ARGs) detected in genomes corresponded well with these observations. Commonly observed ARGs included sul1, sul2, sul3, tet (A), tet (M), qnrS1, qnrB19 and a variety of aminoglycoside-modifying genes, in addition to point mutations in the gyrA and parC genes. Multiple ARGs were predicted to be located on IncN and IncQ1 plasmids of S. Schwarzengrund and S. Muenster, and most qnrB19 genes were carried by Col (pHAD28) plasmids. Seventy-two percent (19/24) of S. Kentucky strains carried multidrug ARGs located in two distinct variants of Salmonella genomic island I. The majority of strains carried full SPI-1 and SPI-2 islands, suggesting full virulence potential
PB MDPI
YR 2021
FD 2021
LK http://hdl.handle.net/10347/24465
UL http://hdl.handle.net/10347/24465
LA eng
NO Antibiotics 2021, 10(2), 99; https://doi.org/10.3390/antibiotics10020099
NO This work was part-supported by an African Research Leader Award to INO from the UK Medical Research Council (MRC) and the UK Department for International Development (DFID) under the MRC/DFID Concordat agreement that is also part of the EDCTP2 programme supported by the European Union. VG acknowledges Consellería de Cultura, Educación e Ordenación Universitaria, Xunta de Galicia for her post-doctoral grant (Grant Number ED481B-2018/018)
DS Minerva
RD 27 abr 2026