RT Journal Article
T1 Molecular identification of zoonotic Rickettsia species in Ixodidae parasitizing wild lagomorphs from Mediterranean ecosystems
A1 Remesar Alonso, Susana
A1 Castro-Scholten, Sabrina
A1 Cano-Terriza, David
A1 Díaz Fernández, Pablo
A1 Morrondo Pelayo, María Patrocinio
A1 Jiménez-Martín, Débora
A1 Rouco Zufiaurre, Carlos
A1 García-Bocanegra, Ignacio
K1 Iberian hare
K1 Rickettsia africae
K1 Rickettsia slovaca
K1 Spain
K1 Wild rabbit
K1 Zoonotic
AB A survey study was carried out to identify tick species parasitizing wild lagomorphs in Mediterranean ecosystems in southern Spain and to determine the occurrence of Rickettsia species present in these ticks in this region. A total of 1304 European wild rabbits (Oryctolagus cuniculus) and 58 Iberian hares (Lepus granatensis) were individually examined for the presence of ticks. Ticks were found in 42.9% and 50% of the wild rabbits and hares sampled, respectively. A total of 1122 ticks were collected and five species, including Rhipicephalus pusillus, Hyalomma lusitanicum, Haemaphysalis hispanica, Ixodes ventalloi and Rhipicephalus sanguineus sensu lato (s.l.), were microscopically and molecularly identified at the 16S rRNA gene. This is the first study on Ixodidae parasitizing Iberian hares. The presence of Rickettsia DNA was assessed in 254 tick pools (according to hunting states, lagomorph species, tick species and tick development stage) using PCR assays targeting the rOmpA, rOmpB and gltA. Twenty-seven pools (10.6%) were positive to Rickettsia DNA. Five zoonotic Rickettsia species were identified, being Rickettsia massiliae the most frequent (4.7%), followed by Rickettsia sibirica subsp. mongolitimonae (2.8%), Rickettsia slovaca (2.0%), Rickettsia aeschlimannii (0.8%) and Rickettsia africae (0.4%). The results suggest that wild rabbits and Iberian hares are parasitized by a wide range of tick species and that these lagomorphs may play an important role in the sylvatic cycle of some zoonotic Rickettsia species in Mediterranean ecosystems. Our data represent the first report of R. massiliae, R. aeschlimannii, R. slovaca and R africae in ticks collected in wild lagomorphs in Europe, and the first report of not imported R. africae in this continent. Since R. slovaca and R. africae DNA was detected in tick species different to their main vectors, further studies are warranted to unravel the role of wild lagomorphs in the epidemiology of these vector-borne pathogens
PB Wiley
YR 2021
FD 2021
LK http://hdl.handle.net/10347/29136
UL http://hdl.handle.net/10347/29136
LA eng
NO Transbound Emerg Dis. 2022;69:e992–e1004. https://doi.org/10.1111/tbed.14379
NO This work has benefited from the financial aid of research grants funded by the Spanish Ministry of Science and Innovation (PID2019-111080RB-C21) and by the University of Córdoba (UCO-FEDER-1264967). S. Castro-Scholten is supported by an FPU grant from the Spanish Ministry of Science, Innovation, and Universities (FPU19/06026). D. Jiménez-Martín holds a I+D+i contract from the University of Cordoba co-supported by the European Social Fund
DS Minerva
RD 24 abr 2026