RT Journal Article
T1 Direct protein quantification in complex sample solutions by surface-engineered nanorod probes
A1 Schrittwieser, Stefan
A1 Pelaz García, Beatriz
A1 Parak, Wolfgang J.
A1 Lentijo-Mozo, Sergio
A1 Soulantica, Katerina
A1 Dieckhoff, Jan
A1 Ludwig, Frank
A1 Schotter, Joerg
K1 Biosensors
K1 Molecular medicine
K1 Nanoparticles
K1 Nanoscale biophysics
AB Detecting biomarkers from complex sample solutions is the key objective of molecular diagnostics. Being able to do so in a simple approach that does not require laborious sample preparation, sophisticated equipment and trained staff is vital for point-of-care applications. Here, we report on the specific detection of the breast cancer biomarker sHER2 directly from serum and saliva samples by a nanorod-based homogeneous biosensing approach, which is easy to operate as it only requires mixing of the samples with the nanorod probes. By careful nanorod surface engineering and homogeneous assay design, we demonstrate that the formation of a protein corona around the nanoparticles does not limit the applicability of our detection method, but on the contrary enables us to conduct in-situ reference measurements, thus further strengthening the point-of-care applicability of our method. Making use of sandwich assays on top of the nanorods, we obtain a limit of detection of 110 pM and 470 pM in 10-fold diluted spiked saliva and serum samples, respectively. In conclusion, our results open up numerous applications in direct protein biomarker quantification, specifically in point-of-care settings where resources are limited and ease-of-use is of essence
PB Springer Nature
YR 2017
FD 2017
LK http://hdl.handle.net/10347/19763
UL http://hdl.handle.net/10347/19763
LA eng
NO Schrittwieser, S., Pelaz, B., Parak, W., Lentijo-Mozo, S., Soulantica, K., & Dieckhoff, J. et al. (2017). Direct protein quantification in complex sample solutions by surface-engineered nanorod probes. Scientific Reports, 7(1). doi: 10.1038/s41598-017-04970-5
NO This research was supported by the European Commission FP7 NAMDIATREAM project (EU NMP4-LA-2010–246479), and the German Research Foundation (DFG grant PA 794/25-1)
DS Minerva
RD 24 abr 2026