RT Journal Article
T1 Effects of short- and long-term exposures of humic acid on the Anammox activity and microbial community
A1 Kraiem, Khadija
A1 Wahab, Mohamed Ali
A1 Kallali, Hamadi
A1 Fra Vázquez, Andrea
A1 Pedrouso Fuentes, Alba
A1 Mosquera Corral, Anuska
A1 Jedidi, Naceur
K1 Anammox bacteria
K1 Heterotrophic bacteria
K1 Humic acid
K1 Inhibition
K1 Nitrogen
AB Humic acid has a controversial effect on the biological treatment processes. Here, we have investigated humic acid effects on the Anammox activity by studying the nitrogen removal efficiencies in batch and continuous conditions and analyzing the microbial community using Fluorescence in situ hybridization (FISH) technique. The results showed that the Anammox activity was affected by the presence of humic acid at a concentration higher than 70 mg/L. In fact, in the presence of humic acid concentration of 200 mg/L, the Anammox activity decreased to 57% in batch and under continuous condition, the ammonium removal efficiencies of the reactor decreased from 78 to 41%. This reduction of Anammox activity after humic acid addition was highlighted by FISH analysis which revealed a considerable reduction of the abundance of Anammox bacteria and the bacteria living in symbiosis with them. Furthermore, a total inhibition of Candidatus Brocadia fulgida was observed. However, humic acid has promoted heterotrophic denitrifying bacteria which became dominant in the reactor. In fact, the evolution of the organic matter in the reactor showed that the added humic acid was used as carbon source by heterotrophic bacteria which explained the shift of metabolism to the favor of heterotrophic denitrifying bacteria. Accordingly, humic acid should be controlled in the influent to avoid Anammox activity inhibition.
PB Springer
SN 0944-1344
YR 2019
FD 2019
LK http://hdl.handle.net/10347/29377
UL http://hdl.handle.net/10347/29377
LA eng
NO Environmental Science and Pollution Research (2019) 26:19012–19024 https://doi.org/10.1007/s11356-018-2786-4
NO This research work is financially supported by the Tunisian Ministry of Higher Education, Scientific Research and Technology
DS Minerva
RD 23 abr 2026