RT Journal Article
T1 Combined Effect of Caspase-Dependent and Caspase-Independent Apoptosis in the Anticancer Activity of Gold Complexes with Phosphine and Benzimidazole Derivatives
A1 Rouco Méndez, Lara
A1 Sánchez González, María Ángeles
A1 Alvariño Romero, Rebeca
A1 Alfonso Rancaño, María Amparo
A1 Vázquez López, Ezequiel M.
A1 García Martínez, Emilia
A1 Maneiro Maneiro, Marcelino
K1 Gold(I) compounds
K1 Cytotoxic activity
K1 Caspase
K1 Apoptosis
K1 Neuroblastoma SH-SY5Y
AB Since the potential anticancer activity of auranofin was discovered, gold compounds have attracted interest with a view to developing anticancer agents that follow cytotoxic mechanisms other than cisplatin. Two benzimidazole gold(I) derivatives containing triphenylphosphine (Au(pben)(PPh3)) (1) or triethylphosphine (Au(pben)(PEt3)) (2) were prepared and characterized by standard techniques. X-ray crystal structures for 1 and 2 were solved. The cytotoxicity of 1 and 2 was tested in human neuroblastoma SH-SY5Y cells. Cells were incubated with compounds for 24 h with concentrations ranging from 10 µM to 1 nM, and the half-maximal inhibitory concentration (IC50) was determined. 1 and 2 showed an IC50 of 2.7 and 1.6 µM, respectively. In order to better understand the type of cell death induced by compounds, neuroblastoma cells were stained with Annexin-FITC and propidium iodide. The fluorescence analysis revealed that compounds were inducing apoptosis; however, pre-treatment with the caspase inhibitor Z-VAD did not reduce cell death. Analysis of compound effects on caspase-3 activity and reactive oxygen species (ROS) production in SH-SY5Y cells revealed an antiproliferative ability mediated through oxidative stress and both caspase-dependent and caspase-independent mechanisms
PB MDPI
YR 2021
FD 2021
LK http://hdl.handle.net/10347/24196
UL http://hdl.handle.net/10347/24196
LA eng
NO Rouco, L.; Sánchez-González, Á.; Alvariño, R.; Alfonso, A.; Vázquez-López, E.M.; García-Martínez, E.; Maneiro, M. Combined Effect of Caspase-Dependent and Caspase-Independent Apoptosis in the Anticancer Activity of Gold Complexes with Phosphine and Benzimidazole Derivatives. Pharmaceuticals 2021, 14, 10
NO The research leading to these results has received funding from the following FEDER cofunded-grants. From Consellería de Cultura, Educación e Ordenación Universitaria, Xunta de Galicia, 2017 GRC GI-1682 (ED431C 2017/01), 2018 GRC GI-1584 (ED431C 2018/13), MetalBIO Network (ED431D 2017/01). From CDTI (Centro para el Desarrollo Técnico Industrial) and Technological Funds, supported by Ministerio de Economía, Industria y Competitividad, CTQ2015-65707-C2-2-P, AGL2016-78728-R (AEI/FEDER, UE), ISCIII/PI16/01830 and RTC-2016-5507-2, ITC-20161072. From European Union POCTEP 0161-Nanoeaters -1-E-1, Interreg AlertoxNet EAPA-317-2016, Interreg Agritox EAPA-998-2018, and H2020 778069-EMERTOX
DS Minerva
RD 23 abr 2026