RT Journal Article
T1 Ultrasound-assisted enzymatic indirect determination of total 3-monochloropropane-1,2-diol esters in canned fish oil fraction
A1 Custodio Mendoza, Jorge Antonio
A1 Ramos, Andrea
A1 Lorenzo, Rosa Antonia
A1 Carro Díaz, Antonia María
K1 3-MCPD
K1 Burkholderia cepacia lipase
K1 Enzymatic transesterification
K1 Experimental design
K1 Fatty acid esters
K1 GC–MS
K1 Canned fish oil
AB A novel, fast, and cost-effective indirect enzymatic method was successfully developed to assess the total 3-monochloropropane-1,2-diol (3-MCPD) in canned food's oil fraction by the action of Burkholderia cepacia lipase. The total 3-MCPD were derivatized with n-Heptafluorobutyrylimidazole (HFBI) for GC–MS analysis during dispersive liquid–liquid microextraction (DLLME). An asymmetrical 2213//8 screening design was used to study the influence of critical factors on the method's effectiveness. The analytical features of the proposed method were assessed following Food and Drug Administration (FDA) guidelines using extra virgin olive oil (EVOO) as a blank sample. Outstanding results were achieved in terms of linearity (r2 = 0.9995), sensitivity, precision (2.1 % to 10.4 % RSD), and accuracy (98.7 % ≤ recovery ≤ 101.9 %). Method efficacy was tested by comparing the results of 10 edible oils for total 3-MCPD with those reported in previous works. A total of 41 samples were analyzed. The lowest 3-MCPD content was found in samples of albacore canned in EVOO oil, while the highest amounts were found in albacore, mackerel, and Atlantic saury samples, all preserved in refined sunflower oil
PB Elsevier
YR 2023
FD 2023
LK http://hdl.handle.net/10347/29396
UL http://hdl.handle.net/10347/29396
LA eng
NO Food Chemistry 403 (2023) 134423. https://doi.org/10.1016/j.foodchem.2022.134423
NO This work was supported by the Spanish Ministry of Science, Innovation, and Universities (Project RTI2018‐096450‐B‐I00) and FEDER funds
DS Minerva
RD 28 abr 2026