RT Journal Article
T1 Genomic hatchery introgression in brown trout (salmo trutta L.): Development of a diagnostic SNP panel for monitoring the impacted mediterranean rivers
A1 Casanova, Adrián
A1 Heras, Sandra
A1 Abras, Alba
A1 Roldán, María Inés
A1 Bouza Fernández, María Carmen
A1 Vera Rodríguez, Manuel
A1 García Marín, José Luis
A1 Martínez Portela, Paulino
K1 2b-RADseq
K1 Brown trout
K1 DNA sequencing
K1 Endangered & extinct species
K1 Gene Pool
K1 Hatcheries
K1 Hybridization
K1 Microsatellites
K1 Mitochondrial DNA
K1 Single Nucleotide
K1 Salmo trutta
K1 Single-nucleotide polymorphism
K1 SNP genotyping
K1 Trout - genetics
AB Brown trout ( L.) populations have been restocked during recent decades to satisfy angling demand and counterbalance the decline of wild populations. Millions of fertile brown trout individuals were released into Mediterranean and Atlantic rivers from hatcheries with homogeneous central European stocks. Consequently, many native gene pools have become endangered by introgressive hybridization with those hatchery stocks. Different genetic tools have been used to identify and evaluate the degree of introgression starting from pure native and restocking reference populations (e.g., * locus, microsatellites). However, due to the high genetic structuring of brown trout, the definition of the "native pool" is hard to achieve. Additionally, although the * locus is useful for determining the introgression degree at the population level, its consistency at individual level is far from being accurate, especially after several generations were since releases. Accordingly, the development of a more powerful and cost-effective tool is essential for an appropriate monitoring to recover brown-trout-native gene pools. Here, we used the 2b restriction site-associated DNA sequencing (2b-RADseq) and Stacks 2 with a reference genome to identify single-nucleotide polymorphisms (SNPs) diagnostic for hatchery-native fish discrimination in the Atlantic and Mediterranean drainages of the Iberian Peninsula. A final set of 20 SNPs was validated in a MassARRAY® System genotyping by contrasting data with the whole SNP dataset using samples with different degree of introgression from those previously recorded. Heterogeneous introgression impact was confirmed among and within river basins, and was the highest in the Mediterranean Slope. The SNP tool reported here should be assessed in a broader sample scenario in Southern Europe considering its potential for monitoring recovery plans.
PB MDPI
YR 2022
FD 2022
LK http://hdl.handle.net/10347/32500
UL http://hdl.handle.net/10347/32500
LA eng
NO Casanova, A., Heras, S., Abras, A., Roldán, M. I., Bouza, C., Vera, M., García-MarínJ.L. & Martínez, P. (2022). Genomic hatchery introgression in brown trout (salmo trutta L.): Development of a diagnostic SNP panel for monitoring the impacted mediterranean rivers. Genes, 13(2), 255. https://doi.org/10.3390/genes13020255
NO This research was funded by Ministerio de Ciencia e Innovación (Spain) through the programState Program for I+D+i oriented to the challenges of society (Project reference, RTI2018-095760-B-C21), Girona University (MPCUdG2016/060) and Consolidation and Structuring of competitiveresearch units of Xunta de Galicia (ED431C 2018/29) projects. Adrián Casanova was a Xunta deGalicia predoctoral fellowship (ED481A-2017/091).
DS Minerva
RD 1 may 2026