RT Journal Article
T1 The first evidence of global meat phosphoproteome changes in response to pre-slaughter stress
A1 Mato Montero, Ariadna
A1 Rodríguez Vázquez, Raquel
A1 López-Pedrouso, María
A1 Bravo López, Susana Belén
A1 Franco Ruiz, Daniel
A1 Zapata Babío, José Carlos
K1 Bos taurus
K1 DFD meat
K1 Meat phosphoproteome
K1 Beef quality
K1 Meat tenderness
K1 Pre-slaughter stress biomarkers
K1 Post-mortem metabolism
AB Pre-slaughter stress (PSS) impairs animal welfare and meat quality. Dark, firm and dry (DFD) are terms used to designate poor quality meats induced by PSS. Protein phosphorylation can be a potentially significant mechanism to explain rapid and multiple physiological and biochemical changes linked to PSS-dependent muscle-to-meat conversion. However, the role of reversible phosphorylation in the response to PSS is still little known. In this study, we report a comparative phosphoproteomic analysis of DFD and normal meats at 24 h post-mortem from the longissimus thoracis (LT) bovine muscle of male calves of the Rubia Gallega breed. For this purpose, two-dimensional gel electrophoresis (2-DE), in-gel multiplex identification of phosphoproteins with PRO-Q Diamond phosphoprotein-specific stain, tandem (MALDI-TOF/TOF) mass spectrometry (MS), novel quantitative phosphoproteomic statistics and bioinformatic tools were used
PB BMC
YR 2019
FD 2019
LK http://hdl.handle.net/10347/21310
UL http://hdl.handle.net/10347/21310
LA eng
NO Mato, A., Rodríguez-Vázquez, R., López-Pedrouso, M. et al. The first evidence of global meat phosphoproteome changes in response to pre-slaughter stress. BMC Genomics 20, 590 (2019). https://doi.org/10.1186/s12864-019-5943-3
NO Mass spectrometry analysis, writing of the manuscript and article-processing charges were supported by grant RTA 2014–00034-C04 from the Instituto Nacional de Investigación y Tecnología Agraria (INIA, Spain). Meat samples were obtained by a project FEADER 2010–04 (Consellería de Medio Rural of Xunta de Galicia, Spain)
DS Minerva
RD 1 may 2026