RT Journal Article
T1 Interspecies transmission between Solea senegalensis and Sparus aurata of reassortant Nervous Necrosis Virus (NNV) strains and effect of stress on the outcome of the infection
A1 Vázquez Salgado, Lucía
A1 Olveira Hermida, José Gabriel
A1 Pereira Dopazo, Carlos
A1 Bandín Matos, Isabel
K1 RGNNV/SJNNV
K1 Senegalese sole
K1 Gilthead seabream
K1 Interspecies transmission
K1 Stress
K1 hsp70 gene
AB Viral Encephalopathy and Retinopathy (VER) episodes in Southern Europe have led to the isolation of several reassortant NNV strains from different fish species, including Senegalese sole and gilthead seabream. Polyculture of both species is being developed to optimize available resources. However, this farming technique can be threatened by NNV horizontal transmission between diseased fish or asymptomatic carriers and non-infected individuals from both fish species, which could lead to a VER outbreak in the facility and seriously affect fish production. Therefore, in this study we have assessed the susceptibility of gilthead seabream and Senegalese sole to two reassortant NNV strains isolated from each of the two fish species, and the possibility of interspecies transmission by cohabiting infected and naïve individuals. Our results showed that both NNV isolates caused moderate mortality rates and replicated in both fish species. In the cohabitation challenges, infective NNV particles were recovered from naïve cohabitants, demonstrating interspecies transmission from infected individuals that shed NNV into the water column. In addition, cumulative mortality in sole cohabitants was significantly higher, presumably due to the stress provoked by the aggressiveness of gilthead seabream. This is supported by the analysis of the hsp70 gene, a stress biomarker overexpressed in the sole cohabitants, especially in those that died on the first day of cohabitation. Therefore, despite the numerous advantages of polyculture, the risk of VER outbreaks represents a serious constraint for the implementation of this technique in Mediterranean aquacultur
PB Elsevier
YR 2022
FD 2022
LK http://hdl.handle.net/10347/27334
UL http://hdl.handle.net/10347/27334
LA eng
NO Aquaculture 547 (2022) 737519
NO This research was funded by the Ministerio de Ciencia, Innovación y Universidades (MCIUI), the Agencia Estatal de Investigación (AEI) and FEDER under Grant RTI2018-094687-B-C21 and also by the Interreg VA Spain-Portugal cooperation program (POCTEP) 2014-2020, 0474_BLUEBIOLAB project, co-funded by FEDER with a research contract granted to L. Vázquez- Salgado
DS Minerva
RD 4 may 2026