RT Dissertation/Thesis
T1 Nanotechnology applied to translational oncology: Developing tools for liquid biopsy
A1 Carmona Ule, Nuria
K1 Liquid biopsy
K1 breast cancer
K1 nanotechnology
K1 CTCs
AB Liquid biopsy represents a powerful tool to support precision medicine,allowing the study of the subset of circulating components that derived from cancer tissue. Among all thesecirculating materials, the Circulating tumour cells (CTCs) represent one of the most promising biomarkers.However, the evaluation of CTCs has not been incorporated yet into current clinical guidelines for treatmentdecision. This might be due to CTCs are infrequent, appearing at an estimated level of one against the backgroundof millions of surrounding normal peripheral mononuclear blood cells (PBMCs). The objective of this thesis projectis to develop innovative nanoparticles that can address two of the critical points that make challenging the use ofCTCs in translational studies of breast cancer: ex vivo culture and isolation. Nanoemulsions composed by acombination of lipids with potential to improve cell viability were formulated. The use of proliferative nanoemulsions(NEs) was successfully translated to ex vivo CTC cultures from metastatic breast cancer patients to expand thesecells for their characterization. The analysis of these cells in culture not only showed that the precursor cells hadmesenchymal and stem features but also it was determined that the capability of CTCs to grow ex vivo using theestablished protocol is a predictive factor in metastatic breast cancer. Finally, the NEs were functionalized withpeptides (Pept-NEs) to endow them with specific recognition capabilities and it was confirmed that Pept-NEs canbe immobilized on surfaces for their use as a potential isolation system.
YR 2022
FD 2022
LK http://hdl.handle.net/10347/28056
UL http://hdl.handle.net/10347/28056
LA eng
DS Minerva
RD 24 abr 2026