RT Journal Article
T1 Multifunctional hybrid chitosan/κ-carrageenan sponges integrating engineered SBA-15@Fe3O4 composites and nano-hydroxyapatite for bone tissue engineering
A1 Vargas Osorio, Zulema
A1 García Acevedo, Pelayo
A1 Piñeiro Redondo, Yolanda
A1 Michálek, Martin
A1 Luzardo Álvarez, Asteria María
A1 Otero Espinar, Francisco Javier
A1 Boccaccini, Aldo Roberto
A1 Rivas Rey, José
K1 Multifunctional hybrid platforms integrating magnetic hyperthermia
K1 Drug delivery and shape recovery functionalities
K1 BDDE-crosslinked and KCl-reticulated chitosan/κ-carrageenan/engineered SBA-15/Fe3O4 composites/nano-sized HAp
K1 Bioactive sponges with remarkable hierarchical porosity and surface roughness for bone tissue engineering
AB Conventional therapies often struggle to overcome key challenges associated with bone tissue disorders that demand advanced and sustained therapy solutions. This underscores the urgent need for multifunctional platforms that combine diagnostic and therapeutic functions with bioactive, degradable, and mechanically robust components. Herein, BDDE-crosslinked chitosan/κ-carrageenan sponges incorporating engineered SBA-15/Fe3O4 composites and nano-sized hydroxyapatite (nHAp) crystals were fabricated and investigated. The resulting hybrid sponges exhibited full shape recovery after mechanical compression under wet conditions and a remarkable absorption capacity without compromising their porous structure, attributed to strong structural integrity. The encapsulated SBA-15/Fe3O4 particles imparted hierarchical porosity and significant surface roughness, enabling high loading (≥150 mg/g) of simvastatin, a drug with potential to enhance bone regeneration, as well as its controlled release over extended periods ≥30 days. Additionally, these engineered composites conferred magnetic hyperthermia functionality, achieving specific absorption rates (SAR) ranging from 1.82 W·g−1 to 22.44 W·g−1 when applied a magnetic field of 28 mT at different kHz, providing them with the ability to modulate the heat response. The incorporation of nHAp into the sponge formulation enhanced both their bioactivity when tested in simulated physiological media, and cell adhesion and proliferation, as confirmed by in vitro direct and indirect contact assays. Cytocompatibility assessments using mouse macrophage (RAW 264.7), human osteosarcoma (MG-63), and preosteoblast (MC3T3-E1) cell lines demonstrated ≥80% viability across all models, revealing the highest proliferation in direct contact. These synergistic and versatile sponges hold promise for applications in bone tissue engineering.
PB Elsevier
SN 0141-8130
YR 2026
FD 2026-04
LK https://hdl.handle.net/10347/46771
UL https://hdl.handle.net/10347/46771
LA eng
NO Vargas-Osorio, Z., García-Acevedo, P., Piñeiro, Y., Michálek, M., Luzardo-Álvarez, A., Otero-Espinar, F. J., Boccaccini, A. R., & Rivas, J. (2026). Multifunctional hybrid chitosan/κ-carrageenan sponges integrating engineered SBA-15@Fe3O4 composites and nano-hydroxyapatite for bone tissue engineering. International Journal of Biological Macromolecules, 353, 151201. 10.1016/j.ijbiomac.2026.151201
NO This work has received funding from the European Union's Horizon Europe research and innovation programme under grant agreement No 101087154, project GlaCerHub. Views and opinions expressed are however those of the author(s) only and do not necessarily reflect those of the European Union or the European Union or European Research Executive Agency (REA). Neither the European Union nor the granting authority can be held responsible for them. This research was funded by SIBILA project with grant APVV-23-0097 (Agentúra na podporu výskumu a vývoja). P. García-Acevedo thanks to Axencia Galega de Innovación (Spain) for his Posdoctoral Grant (Axudas de apoio á etapa de formación posdoutoral - IN606B-2024.1).
DS Minerva
RD 18 abr 2026