RT Journal Article
T1 Context-dependent impact of RAS oncogene expression on cellular reprogramming to pluripotency
A1 Ferreirós, Alba
A1 Pedrosa, Pablo
A1 Silva Álvarez, Sabela da
A1 Triana Martínez, Francisco
A1 Vilas Martínez, Jéssica María
A1 Picallos Rabina, Pilar
A1 González, Patricia
A1 Gómez, María
A1 Li, Han
A1 García-Caballero Parada, Tomás
A1 González Barcia, Miguel
A1 Vidal Figueroa, Anxo
A1 Collado Rodríguez, Manuel
K1 Ras
K1 Oncogenes
K1 Cell reprogramming
K1 iPSC
K1 Cell plasticity
AB Induction of pluripotency in somatic cells with defined genetic factors has been successfully used to investigate the mechanisms of disease initiation and progression. Cellular reprogramming and oncogenic transformation share common features; both involve undergoing a dramatic change in cell identity, and immortalization is a key step for cancer progression that enhances reprogramming. However, there are very few examples of complete successful reprogramming of tumor cells. Here we address the effect of expressing an active oncogene, RAS, on the process of reprogramming and found that, while combined expression with reprogramming factors enhanced dedifferentiation, expression within the context of neoplastic transformation impaired reprogramming. RAS induces expression changes that promote loss of cell identity and acquisition of stemness in a paracrine manner and these changes result in reprogramming when combined with reprogramming factors. When cells carry cooperating oncogenic defects, RAS drives cells into an incompatible cellular fate of malignancy. 
PB Cell Press
YR 2019
FD 2019
LK http://hdl.handle.net/10347/21396
UL http://hdl.handle.net/10347/21396
LA eng
NO Ferreirós, A., Pedrosa, P., Da Silva-Álvarez, S., Triana-Martínez, F., Vilas, J., Picallos-Rabina, P., González, P., Gómez, M., Li, H., García-Caballero, T., González-Barcia, M., Vidal, A. and Collado, M., 2019. Context-Dependent Impact of RAS Oncogene Expression on Cellular Reprogramming to Pluripotency. Stem Cell Reports, 12(5), pp.1099-1112.
NO A.F. is an FPU predoctoral fellow from MECD; P.P. and J.M.V. are predoctoral fellows from Xunta de Galicia; F.T.-M. is a postdoctoral fellow from CONACYT (cvu 268632). M.C. is a ‘‘Miguel Servet II’’ investigator (CPII16/00015). Work in the laboratory of M.C. is funded by an ISCIII and EU-FEDER grant (PI14/00554). Work in the laboratory of A.V. is funded by Xunta de Galicia (ED431B 2016) and MINECO (MAT2017-89678-R; cofinanced with FEDER Funds)
DS Minerva
RD 23 abr 2026