RT Journal Article
T1 Evaluating Commercial Loop-Mediated Isothermal Amplification Master Mixes for Enhanced Detection of Foodborne Pathogens
A1 Costa Ribeiro, Ana
A1 Lamas Freire, Alexandre
A1 Garrido Maestu, Alejandro
K1 loop-mediated isothermal amplification
K1 LAMP
K1 foodborne pathogens
K1 L. monocytogenes
K1 Salmonella spp.
K1 E. coli O157
K1 master mix
AB Loop-mediated isothermal amplification, LAMP, is nowadays the most popular isothermal nucleic acid amplification technique, and as such, several commercial, ready-to-use master mixes have flourished. Unfortunately, independent studies to determine their performance are limited. The current study performed an independent evaluation of the existing ready-to-use commercial LAMP master mixes WarmStart® LAMP Kit, LavaLAMP™ DNA Master Mix, Saphir Bst Turbo GreenMaster, OptiGene Fast Master Mix ISO-004, and SynLAMP Mix. To reduce bias, three different genes, namely ttr (Salmonella spp.), rfbE (E. coli O157), and hly (Listeria monocytogenes), were targeted. The comparison was based on amplification speed, performance with decreasing DNA concentrations, and the effect of five typical LAMP reaction additives (betaine, DMSO, pullulan, TMAC, and GuHCl). Significant differences were observed among the different master mixes. OptiGene provided the fastest amplification and showed less detrimental effects associated with the supplements evaluated. Out of the chemicals tested, pullulan provided the best results in terms of amplification speed. It is noteworthy that the different additives impacted the master mixes differently. Overall, the current study provides insights into the performance of commercial LAMP master mixes, which can be of value for the scientific community to better select appropriate reagents when developing new methods.
PB MDPI
SN 2304-8158
YR 2024
FD 2024-05-24
LK https://hdl.handle.net/10347/43521
UL https://hdl.handle.net/10347/43521
LA eng
NO Costa-Ribeiro, A.; Lamas, A.; Garrido-Maestu, A. Evaluating Commercial Loop-Mediated Isothermal Amplification Master Mixes for Enhanced Detection of Foodborne Pathogens. Foods 2024, 13, 1635. https://doi.org/10.3390/ foods13111635
NO Agenda SMARTgNOSTICS—Global Testing & Diagnostics Solutions for antimicrobial resistances”, reference n.º C644915155-00000024
NO Fundação para a Ciência e a Tecnologia through the Scientific Employment Stimulus Program (2021.02810.CEECIND)
DS Minerva
RD 28 abr 2026