RT Journal Article
T1 DNA methylation changes in genes involved in inflammation and depression in fibromyalgia: a pilot study
A1 Gerra, Maria Carla
A1 Carnevali, Davide
A1 Pedersen, Inge Søkilde
A1 Donnini, Claudia
A1 Manfredini, Matteo
A1 González Villar, Alberto Jacobo
A1 Triñanes, Yolanda
A1 Pidal Miranda, Marina
A1 Arendt-Nielsen, Lars
A1 Carrillo de la Peña, María Teresa
K1 Biomarkers
K1 DNA methylation
K1 Epigenetics
K1 Fibromyalgia
AB Objectives: The present pilot study aims to investigateDNA methylation changes of genes related to fibromyalgia(FM) development and its main comorbid symptoms,including sleep impairment, inflammation, depressionand other psychiatric disorders. Epigenetic modificationsmight trigger or perpetuate complex interplay betweenpain transduction/transmission, central pain processingand experienced stressors in vulnerable individuals.Methods: We conducted DNA methylation analysis bytargeted bisulfite NGS sequencing testing differentialmethylation in 112 genomic regions from leukocytes ofeight women with FM and their eight healthy sisters ascontrols.Results: Tests for differentially methylated regions andcytosines brought focus on the GRM2 gene, encoding the metabotropic glutamate receptor2. The slightly increasedDNA methylation observed in the GRM2 region of FM patientsmay confirm the involvement of the glutamatepathway in this pathological condition. Logistic regressionhighlighted the simultaneous association of methylationlevels of depression and inflammation-related geneswith FM.Conclusions: Altogether, the results evidence the glutamatepathway involvement in FM and support the idea thata combination of methylated and unmethylated genescould represent a risk factor to FM or its consequence, morethan single genes. Further studies on the identified biomarkerscould contribute to unravel the causative underlyingFM mechanisms, giving reliable directions toresearch, improving the diagnosis and effective therapies
PB De Gruyter
SN 1877-8879
YR 2021
FD 2021
LK http://hdl.handle.net/10347/27517
UL http://hdl.handle.net/10347/27517
LA eng
NO Gerra, M., Carnevali, D., Pedersen, I., Donnini, C., Manfredini, M., González-Villar, A., Triñanes, Y., Pidal-Miranda, M., Arendt-Nielsen, L. & Carrillo-de-la-Peña, M. (2021). DNA methylation changes in genes involved in inflammation and depression in fibromyalgia: a pilot study. Scandinavian Journal of Pain, 21(2), 372-383
NO This study was supported by Spanish Government Funding (Ministerio de Economía y Competitividad: grant PSI2013-45818-R). The genotyping service was carried out at CEGEN-PRB3-ISCIII; it is supported by grant PT17/0019, of the PE I + D + i 2013–2016, funded by ISCIII and ERDF. MCG and LAN are part of the Center for Neuroplasticity and Pain (CNAP) which is supported by the Danish National Research Foundation (DNRF121)
DS Minerva
RD 24 abr 2026