RT Journal Article
T1 Matrix solid-phase dispersion as a greener alternative to obtain bioactive extracts from Haematococcus pluvialis. Characterization by UHPLC-QToF
A1 Castillo Zamora, Aly Jesús
A1 Pereira, Simón
A1 Otero Casal, Ana María
A1 Fiol López, Sarah
A1 García Jares, Carmen María
A1 Lores Aguín, Marta
AB So far, research on the microalga Haematococcus pluvialis has been focused mainly on the exploitation of its high astaxanthin content, leaving aside the use of other bioactive compounds present. This study is focused on obtaining and characterizing extracts enriched in bioactive compounds from this microalga red aplanospores. This is performed by means of Matrix Solid-Phase Dispersion (MSPD) extraction process, in an environmentally friendly way with low energy consumption and GRAS solvents. The effects of extraction parameters, particularly the extraction solvents (ethanol, ethyl lactate and water) are studied, in order to obtain maximum recovery of the main antioxidant compounds of interest (carotenoids, fatty acids and derivatives). Characterization of extracts is carried out by HPLC-DAD (High Performance Liquid Chromatography Diode Array Detector) and UHPLC-QToF (Ultra High-Performance Liquid Chromatography Quadrupole Time-of-Flight). The results show that MSPD produced extracts with higher bioactive compound recoveries than conventional cell disruption extractions. At the same time, a novel untargeted characterization for this species is performed, identifying compounds not previously dated in H. pluvialis, which include 10-phenyldecanoic acid and the -oxo and -hydroxy derivatives of palmitic acid. This approach, first applied to a freshwater microalgae, characterized by rigid and resistant aplanospores, provided a synergistic and sustainable extract, giving a broader focus on the use of this microalga
PB Royal Society of Chemistry
YR 2020
FD 2020
LK http://hdl.handle.net/10347/23450
UL http://hdl.handle.net/10347/23450
LA eng
NO RSC Adv., 2020,10, 27995-28006
NO This work was supported by project EQC2018-005011-P (Ministry of Science, Innovation and Universities, Spain). The authors belongs to CRETUS Institute and CRETUS Strategic Partnership (ED431E 2018/01) co-funded by FEDER (UE)
DS Minerva
RD 23 abr 2026