RT Journal Article
T1 Morphological Abnormalities and Gene Expression Changes Caused by High Incubation Temperatures in Zebrafish Xenografts with Human Cancer Cells
A1 Cabezas Sáinz, Pablo
A1 Coppel, Carlos
A1 Pensado López, Alba
A1 Fernández García, Pedro
A1 Muinelo Romay, Laura
A1 López López, Rafael
A1 Rubiolo Gaytán, Juan Andrés
A1 Sánchez Piñón, Laura
K1 Zebrafish
K1 Development
K1 Xenotransplantation
K1 Incubation-temperature
K1 Gene-expression
AB Published studies show that most of the human cancer xenograft studies in zebrafish embryos have used incubation temperatures in the range of 32–34 °C for 3–6 days post-injection, trying to find a compromise temperature between the zebrafish embryos (28 °C) and the human injected cells (37 °C). While this experimental setup is widely used, a question remains: is possible to overcome the drawbacks caused by a suboptimal temperature for the injected cells? To clarify the effect of temperature and injected cells on the host, in this study, we analyzed the development and health of the last in response to different temperatures in the presence or absence of injected human cancer cells. Comparing different incubation temperatures (28, 34 and 36 °C), we determined morphological abnormalities and developmental effects in injected and non-injected embryos at different time points. Besides this, the expression of selected genes was determined by qPCR to determine temperature affected metabolic processes in the embryos. The results indicate that an incubation temperature of 36 °C during a period of 48 h is suitable for xenotransplantation without morphological or metabolic changes that could be affecting the host or the injected cells, allowing them to proliferate near their optimal temperature
PB MDPI
YR 2021
FD 2021
LK http://hdl.handle.net/10347/24464
UL http://hdl.handle.net/10347/24464
LA eng
NO Genes 2021, 12(1), 113; https://doi.org/10.3390/genes12010113
NO This research is funded by ‘Consolidación e estruturación de unidades de investigación competitivas do SUG. Grupos de referencia competitiva’ (ED431C 2018/29) and ‘Axuda á formación da etapa predoutoral’ of Xunta de Galicia
DS Minerva
RD 23 abr 2026