RT Journal Article
T1 Field evaluation of the enhanced MM3-COPRO ELISA test for the diagnosis of Fasciola hepatica infection in sheep
A1 Mezo Menéndez, Mercedes
A1 González Warleta, Marta
A1 Castro-Hermida, José Antonio
A1 Martínez Sernández, Victoria
A1 Martínez Ubeira, Florencio César
K1 Bird eggs
K1 Fasciola
K1 Fascioliasis
K1 Sheep
K1 Enzyme-linked immunoassays
K1 Parasitic diseases
K1 Statistical distributions
K1 Diagnostic medicine
AB Fasciolosis is a severe zoonosis responsible for major economic losses in livestock. The enhanced MM3-COPRO test (eMM3-COPRO) and the commercial version BIO K 201 (Bio-X Diagnostics, Rochefort, Belgium) are widely used as immunodiagnostic tools for the specific detection of coproantigens released by Fasciola during the late prepatent and patent stages of infection. However, performance of the eMM3-COPRO has never been evaluated under field conditions. To address this gap, a large number of ovine faecal samples, collected in a region where fasciolosis is endemic (Galicia, NW Spain), were analyzed. Two groups of sheep flocks were selected according to the Fasciola infection status: ‘Fasciola-free’ and ‘Fasciola-infected’ flocks. ‘Fasciola-free’ flocks were seronegative flocks with no history of fasciolosis detected by either coproscopy or necropsy in the last 5 years. Faecal samples from these sheep were used to calculate a cut-off value for infection (OD = 0.021). The cut-off was calculated using a bootstrap resampling method that enables estimation of the sampling distribution of the statistical parameters without making assumptions about the underlying data distribution. ‘Fasciola-infected’ flocks were characterized by high seroprevalence, a history of fasciolosis and periodical treatment with flukicides. Samples from these flocks were used to estimate the diagnostic accuracy of the eMM3-COPRO relative to coproscopy, which although limited by poor sensitivity is the only reference test available for diagnosing fasciolosis in vivo. To overcome this limitation, all animals classified positive by eMM3-COPRO were treated with triclabendazole and then retested. The eMM3-COPRO displayed higher sensitivity than coproscopy, as it detected coproantigens in all samples with positive coproscopy and in 12% of samples with negative coproscopy. The test also proved highly specific as coproantigens disappeared after the treatment. The eMM3-COPRO was less time consuming than coproscopy, particularly when the procedure involved numerous samples, and showed promise as a tool for monitoring flukicide efficacy
PB Public Library of Science
YR 2022
FD 2022
LK http://hdl.handle.net/10347/30137
UL http://hdl.handle.net/10347/30137
LA eng
NO Mezo M, González-Warleta M, Castro-Hermida JA, Martínez-Sernández V, Ubeira FM (2022) Field evaluation of the enhanced MM3-COPRO ELISA test for the diagnosis of Fasciola hepatica infection in sheep. PLOS ONE 17(3): e0265569. https://doi.org/10.1371/journal.pone.0265569
DS Minerva
RD 24 abr 2026