RT Journal Article
T1 Is LRP2 Involved in Leptin Transport over the Blood-Brain Barrier and Development of Obesity?
A1 Sandin, Elvira S.
A1 Folberth, Julica
A1 Müller-Fielitz, Helge
A1 Pietrzik, Claus Ulrich
A1 Herold, Elisabeth
A1 Willnow, Thomas
A1 Pfluger, Paul
A1 Nogueiras Pozo, Rubén
A1 Prevot, Vincent
A1 Krey, Thomas
A1 Schwaninger, Markus
K1 Leptin
K1 Blood-brain barrier
K1 LRP2
K1 ERK1/2
K1 Gaussia luciferase
K1 Fusion protein
AB The mechanisms underlying the transport of leptin into the brain are still largely unclear. While the leptin receptor has been implicated in the transport process, recent evidence has suggested an additional role of LRP2 (megalin). To evaluate the function of LRP2 for leptin transport across the blood-brain barrier (BBB), we developed a novel leptin-luciferase fusion protein (pLG), which stimulated leptin signaling and was transported in an in vitro BBB model based on porcine endothelial cells. The LRP inhibitor RAP did not affect leptin transport, arguing against a role of LRP2. In line with this, the selective deletion of LRP2 in brain endothelial cells and epithelial cells of the choroid plexus did not influence bodyweight, body composition, food intake, or energy expenditure of mice. These findings suggest that LRP2 at the BBB is not involved in the transport of leptin into the brain, nor in the development of obesity as has previously been described
PB MDPI
YR 2021
FD 2021
LK http://hdl.handle.net/10347/26250
UL http://hdl.handle.net/10347/26250
LA eng
NO Int. J. Mol. Sci. 2021, 22(9), 4998; https://doi.org/10.3390/ijms22094998
NO This project has received funding from the Deutsche Forschungsgemeinschaft (GRK1957) to M.S. and the European Research Council (ERC) Synergy Grant-2019-WATCH-810331 to V.P., R.N. and M.S.
DS Minerva
RD 4 may 2026