RT Journal Article
T1 Impedimetric Biosensor Based on a Hechtia argentea Lectin for the Detection of Salmonella spp.
A1 López Téllez, Jorge
A1 Sánchez Ortega, Irais
A1 Hornung Leoni, Claudia Teresa
A1 Santos, Eva María
A1 Miranda López, José Manuel
A1 Rodríguez, José Antonio
K1 Biosensors
K1 Impedance spectroscopy
K1 Lectins
K1 Salmonella
K1 Screen-printed electrodes
AB A sensitive electrochemical detection method for Salmonella spp. was described, based on the use of Hechtia argentea lectin immobilised on a screen-printed gold electrode. The lectin was extracted from Hechtia argentea, a plant belonging to the Bromeliaceae family. The lectin with molecular weight near 27.4 kDa showed selectivity towards D-mannose, contained on the lipopolysaccharide cell wall of Salmonella spp. Carbohydrate selectivity of the lectin was measured as a change in impedance with respect to concentration. The binding of the bacteria to the biosensor surface increased impedance with increasing concentrations of Salmonella spp., achieving a linear range of detection of 15–2.57 × 107 CFU mL−1, with a limit of detection of 5 CFU mL−1. Increases in impedance were measured using electrochemical impedance spectroscopy and analysed using Nyquist plots. The biosensor was applied in analysis of hen egg samples, and the results were consistent with those obtained using the official analysis methodology
PB MDPI
YR 2020
FD 2020
LK http://hdl.handle.net/10347/24063
UL http://hdl.handle.net/10347/24063
LA eng
NO Lopez-Tellez, J.; Sanchez-Ortega, I.; Hornung-Leoni, C.T.; Santos, E.M.; Miranda, J.M.; Rodriguez, J.A. Impedimetric Biosensor Based on a Hechtia argentea Lectin for the Detection of Salmonella spp.. Chemosensors 2020, 8, 115
DS Minerva
RD 24 abr 2026