RT Journal Article
T1 Bee trypanosomatids: first steps in the analysis of the fenetic variation and population structure of Lotmaria passim, Crithidia bombi and Crithidia mellificae
A1 Bartolomé Husson, Carolina
A1 Buendía Abad, María
A1 Ornosa Gallego, Concepción
A1 Rúa Tarín, María del Pilar de la
A1 Martín Hernández, Raquel
A1 Higes Pascual, Mariano
A1 Maside Rodríguez, Xulio Manuel
K1 Population genetics
K1 Genetic diversity
K1 Population structure
K1 Lotmaria passim
K1 Crithidia mellificae
K1 Crithidia bombi
AB Trypanosomatids are among the most prevalent parasites in bees but, despite the fact that their impact on the colonies can be quite important and that their infectivity may potentially depend on their genotypes, little is known about the population diversity of these pathogens. Here we cloned and sequenced three non-repetitive single copy loci (DNA topoisomerase II, glyceraldehyde-3-phosphate dehydrogenase and RNA polymerase II large subunit, RPB1) to produce new genetic data from Crithidia bombi, C. mellificae and Lotmaria passim isolated from honeybees and bumblebees. These were analysed by applying population genetic tools in order to quantify and compare their variability within and between species, and to obtain information on their demography and population structure. The general pattern for the three species was that they were subject to the action of purifying selection on nonsynonymous variants, the levels of within species diversity were similar irrespective of the host, there was evidence of recombination among haplotypes and they showed no haplotype structuring according to the host. C. bombi exhibited the lowest levels of synonymous variation (πS= 0.06 ± 0.04%) — and a mutation frequency distribution compatible with a population expansion after a bottleneck — that contrasted with the extensive polymorphism displayed by C. mellificae (πS= 2.24 ± 1.00 %), which likely has a more ancient origin. L. passim showed intermediate values (πS= 0.40 ± 0.28 %) and an excess of variants a low frequencies probably linked to the spread of this species to new geographical areas.
PB Springer
SN 0095-3628
YR 2022
FD 2022
LK http://hdl.handle.net/10347/30062
UL http://hdl.handle.net/10347/30062
LA eng
NO Microbial Ecology volume 84, pages856–867 (2022)
NO This study was supported by the Ministerio de Economía y Competitividad (MINECO) (grant number CGL2012-34897), the Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) - European Regional Development Fund (ERDF) (grant numbers E-RTA2014-00003-C03-01, 02 and 03), the Eva Crane Trust (grant number ECTA_20210308) and the Fundación Séneca - Agencia de Ciencia y Tecnología de la Región de Murcia (grant of Regional Excellence19908/GERM/2015)
DS Minerva
RD 29 abr 2026