RT Journal Article
T1 Influence of haemolysis on blood biochemistry profiles in cattle
A1 Larrán Franco, Belén
A1 López Alonso, María Marta
A1 Miranda Castañón, Marta Inés
A1 Graña, Almudena
A1 Rigueira Rey, Lucas
A1 Orjales Galdo, Inmaculada
K1 Biochemistry
K1 Cattle
K1 Hemolysis
K1 Interference
K1 Laboratory error
K1 Hypocalcaemia
AB Although haemolysis is the most common source of preanalytical error in clinical laboratories, its influence on cattle biochemistry remains poorly understood. The effect of haemolysis and its clinical relevance were investigated in 70 samples in which haemolysis was artificially induced (by spiking with increasing amounts of haemolysate, yielding 0.0%, 0.2%, 0.5%, 1.0%, 2.5%, 5.0% and 10% haemolysis degree (HD)), focusing on key parameters for bovine metabolic health assessment, including albumin, alkaline phosphatase (ALP), aspartate aminotransferase (AST), blood urea nitrogen (BUN), calcium (Ca), cholesterol, creatinine, creatine kinase (CK), gamma-glutamyl transferase (GGT), globulins, magnesium (Mg), phosphorus (P), total bilirubin (TBIL) and total proteins (TP). Preanalytical haemolysis significantly affected most (8 of 14) of the biochemical parameters analysed, leading to significant increases in concentrations of albumin (starting at 5% HD), cholesterol (at 5% HD) and P (at 10% HD) and to significant decreases in Ca (at 2.5% HD), creatinine (at 5% HD), globulins (at 10% HD), TBIL (at 2.5% HD) and TP (at 10% HD). Comparison of the present and previous data indicated that, for each parameter, the HD required to produce significant bias and the clinical relevance of over- and underestimation are variable and appear to depend on the analytical technique used. Therefore, different laboratories should evaluate the influence of haemolysis in their analytical results and provide advice to clinicians accordingly. Affected parameters should be interpreted together with clinical signs and other analytical data to minimize misinterpretations (false or masked variations). Finally, due to the significant impact on numerous parameters and the limited potential for correction, we recommend rejection of samples with >10% HD
PB Elsevier
SN 0034-5288
YR 2024
FD 2024
LK http://hdl.handle.net/10347/33861
UL http://hdl.handle.net/10347/33861
LA eng
NO Research in Veterinary Science, Volume 171, 2024, 105203
NO This work was supported by laboratory RAL S.A. Belén Larrán is in receipt of a FPU fellowship (Ref. FPU21/01742) from the Spanish Ministry of Education, Culture and Sports. Belén Larrán, Marta Miranda, Marta López-Alonso and Inmaculada Orjales belong to Grupo de Potencial Crecimiento financed by GAIN (Axencia Galega de Innovación; grant number ED431B 2023/008)
DS Minerva
RD 26 abr 2026