RT Journal Article
T1 Use of vivo-morpholinos for gene knockdown in the postnatal shark retina
A1 Rodríguez Arrizabalaga, Mariña
A1 Hernández Núñez, Ismael
A1 Candal Suárez, Eva María
A1 Barreiro Iglesias, Antón
K1 Cartilaginous fish
K1 Retina
K1 Vivo-morpholino
K1 TUNEL
K1 PCNA
K1 pH3
AB Work in the catshark Scyliorhinus canicula has shown that the evolutionary origin of postnatal neurogenesis in vertebrates is earlier than previously thought. Thus, the catshark can serve as a model of interest to understand postnatal neurogenic processes and their evolution in vertebrates. One of the best characterized neurogenic niches of the catshark CNS is found in the peripheral region of the retina. Unfortunately, the lack of genetic tools in sharks limits the possibilities to deepen in the study of genes involved in the neurogenic process. Here, we report a method for gene knockdown in the juvenile catshark retina based on the use of Vivo-Morpholinos. To establish the method, we designed Vivo-Morpholinos against the proliferation marker PCNA. We first evaluated the possible toxicity of 3 different intraocular administration regimes. After this optimization step, we show that a single intraocular injection of the PCNA Vivo-Morpholino decreases the expression of PCNA in the peripheral retina, which leads to reduced mitotic activity in this region. This method will help in deciphering the role of other genes potentially involved in postnatal neurogenesis in this animal model
PB Elsevier
YR 2023
FD 2023
LK http://hdl.handle.net/10347/29667
UL http://hdl.handle.net/10347/29667
LA eng
NO Experimental Eye Research 226 (2023). https://doi.org/10.1016/j.exer.2022.109333
NO Grant PID 2020-115121 GB-I00 funded by MCIN/AEI/10.13039/501100011033 to A. Barreiro-Iglesias. Grant ED 431C 2021/18 funded by Xunta de Galicia to E. Candal
DS Minerva
RD 28 abr 2026