RT Journal Article
T1 In vivo subchronic effects of ciguatoxin-related compounds, reevaluation of their toxicity
A1 Raposo García, Sandra
A1 Boente Juncal, Andrea
A1 Camiña García, María Mercedes
A1 Costas Sánchez, Celia
A1 Cao Cancelas, Alejandro
A1 Louzao Ojeda, María del Carmen
A1 Cifuentes Martínez, José Manuel
A1 Vale González, María del Carmen
A1 Botana López, Luis Miguel
A1 Rodríguez Vieytes, Mercedes
K1 Maitotoxin 1
K1 Ciguatoxin
K1 Gambierone
K1 Maitotoxin 3 (MTX3) or 44-methylgambierone
K1 Ciguatera fish poisoning
K1 In vivo
K1 Acute toxicity
K1 Subchronic toxicity
AB Ciguatoxins are marine compounds that share a ladder-shaped polyether structure produced by dinoflagellates of the genus Gambierdiscus and Fukuyoa, and include maitotoxins (MTX1 and MTX3), ciguatoxins (CTX3C) and analogues (gambierone), components of one of the most frequent human foodborne illness diseases known as ciguatera fish poisoning. This disease was previously found primarily in tropical and subtropical areas but nowadays, the dinoflagellates producers of ciguatoxins had spread to European coasts. One decade ago, the European Food Safety Authority has raised the need to complete the toxicological available data for the ciguatoxin group of compounds. Thus, in this work, the in vivo effects of ciguatoxin-related compounds have been investigated using internationally adopted guidelines for the testing of chemicals. Intraperitoneal acute toxicity was tested for maitotoxin 1 at doses between 200 and 3200 ng/kg and the acute oral toxicity of Pacific Ciguatoxin CTX3C at 330 and 1050 ng/kg and maitotoxin 1 at 800 ng/kg were also evaluated showing not effects on mice survival after a 96 h observation period. Therefore, for the following experiments the oral subchronic doses were between 172 and 1760 ng/kg for gambierone, 10 and 102 ng/kg for Pacific Ciguatoxin CTX3C, 550 and 1760 ng/kg for maitotoxin 3 and 800, 2560 and 5000 ng/kg for maitotoxin 1. The results presented here raise the need to reevaluate the in vivo activity of these agents. Although the intraperitoneal lethal dose of maitotoxin 1 is assumed to be 50 ng/kg, without chemical purity identifications and description of the bioassay procedures, in this work, an intraperitoneal lethal dose of 1107 ng/kg was obtained. Therefore, the data presented here highlight the need to use a common procedure and certified reference material to clearly establish the levels of these environmental contaminants in food
PB Springer
SN 0340-5761
YR 2022
FD 2022
LK http://hdl.handle.net/10347/29169
UL http://hdl.handle.net/10347/29169
LA eng
NO Archives of Toxicology 96, 2621–2638 (2022). https://doi.org/10.1007/s00204-022-03315-0
NO Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. The research leading to these results has received funding from the following FEDER cofunded-grants. From Conselleria de Cultura, Educacion e Ordenación Universitaria, Xunta de Galicia, GRC (ED431C 2021/01). From Ministerio de Ciencia e Innovación PID 2020-11262RB-C21. From European Union Interreg AlertoxNet EAPA-317-2016, Interreg Agritox EAPA-998-2018, and H2020 778069-EMERTOX
DS Minerva
RD 27 abr 2026