RT Journal Article
T1 Effect of culture temperature on the heterologous expression of Pleurotus eryngii versatile peroxidase in Aspergillus hosts
A1 Eibes González, Gemma María
A1 Lu Chau, Thelmo Alejandro
A1 Ruiz-Dueñas, F. J.
A1 Feijoo Costa, Gumersindo
A1 Martínez, M. J.
A1 Martínez, A. T.
A1 Lema Rodicio, Juan Manuel
K1 Aspergillus
K1 Proteases
K1 Temperature
K1 Versatile peroxidase
AB Production of recombinant versatile peroxidase in Aspergillus hosts was optimized through the modification of temperature during bioreactor cultivations. To further this purpose, the cDNA encoding a versatile peroxidase of Pleurotus eryngii was expressed under control of the alcohol dehydrogenase (alcA) promoter of Aspergillus nidulans. A dependence of recombinant peroxidase production on cultivation temperature was found. Lowering the culture temperature from 28 to 19 °C enhanced the level of active peroxidase 5.8-fold and reduced the effective proteolytic activity twofold. Thus, a maximum peroxidase activity of 466 U L-1 was reached. The same optimization scheme was applied to a recombinant Aspergillus niger that bore the alcohol dehydrogenase regulator (alcR), enabling transformation with the peroxidase cDNA under the same alcA promoter. However, with this strain, the peroxidase activity was not improved, while the effective proteolytic activity was increased between 3- and 11-fold compared to that obtained with A. nidulans.
PB Springer Nature
SN 1615-7591
YR 2008
FD 2008-05-15
LK https://hdl.handle.net/10347/39252
UL https://hdl.handle.net/10347/39252
LA eng
NO Eibes, G.M., Lú-Chau, T.A., Ruiz-Dueñas, F.J. et al. Effect of culture temperature on the heterologous expression of Pleurotus eryngii versatile peroxidase in Aspergillus hosts. Bioprocess Biosyst Eng 32, 129–134 (2009). https://doi.org/10.1007/s00449-008-0231-7
DS Minerva
RD 27 abr 2026