RT Journal Article
T1 Transcriptome changes in response to temperature in the fish pathogen Photobacterium damselae subsp. damselae: Clues to understand the emergence of disease outbreaks at increased seawater temperatures
A1 Matanza Fente, José Manuel
A1 Rodríguez Osorio, Carlos
K1 Photobacterium damselae
K1 Fish pathogen
K1 Virulence factors
K1 Gene expression
K1 Fish farming
K1 Serum proteins
K1 Chaperone proteins
K1 Ocean temperature
K1 Secretion systems
K1 Toxins
AB The marine bacterium Photobacterium damselae subsp. damselae (Pdd) is a generalist andfacultative pathogen that causes disease in a wide range of marine animals including fishspecies of importance in aquaculture. Disease outbreaks in fish farms have been correlatedwith an increased water temperature during summer months. In this study, we have usedRNA sequencing to analyze the transcriptome of Pdd RM-71 cultured at two different temperatures, which simulated temperature conditions experienced during free swimming lifestyle at mid latitudes in winter months (15˚C) and during outbreaks in aquaculture in warmsummer months (25˚C). The enhanced bacterial growth of Pdd observed at 25˚C in comparison to 15˚C suggests that an elevated seawater temperature contributes to the build-up of asufficient bacterial population to cause disease. In comparison to growth at 15˚C, growth at25˚C resulted in the upregulation of genes involved in DNA synthesis, nutrient uptake, chemotaxis, flagellar motility, secretion systems and antimicrobial resistance. Plasmid-encodedvirulence factors, which include a putative adhesin/invasin OmpU, a transferrin receptorand a serum resistance protein, were also upregulated. Transcription factor RpoS, genesinvolved in cold shock response, modulation of cell envelope and amino acid metabolism,as well as genes of yet unknown function were downregulated at 25˚C. Notably, the geneencoding damselysin cytotoxin (Dly) was among the most highly transcribed genes at thetwo assayed temperatures, at levels comparable to the most highly expressed housekeeping genes. This study contributes to our understanding of the regulatory networks and biology of a generalist marine bacterial pathogen, and provides evidence that temperatureregulates multiple physiological and virulence-related functions in Pdd
PB Plos
YR 2018
FD 2018-12
LK http://hdl.handle.net/10347/20022
UL http://hdl.handle.net/10347/20022
LA eng
NO Matanza XM, Osorio CR (2018) Transcriptome changes in response to temperature in the fish pathogen Photobacterium damselae subsp. damselae: Clues to understand the emergence of disease outbreaks at increased seawater temperatures. PLoS ONE 13(12): e0210118. https://doi.org/10.1371/journal.pone.0210118
NO This work has been supported by grant AGL2016-79738-R (AEI/FEDER, EU) from the State Agency for Research (AEI) of Spain, and co-funded by the FEDER Programme from the European Union, to CRO. The support of Xunta de Galicia (Spain) with grant GRC-2014/007 to CRO and XMM is also acknowledged. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Funders websites: http://www.ciencia.gob.es/, http://www.edu.xunta.gal/portal/
DS Minerva
RD 22 abr 2026