RT Journal Article
T1 Mechanisms of Interferon- -Induced Survival in Fetal and Neonatal Primary Astrocytes
A1 Barca Mayo, Olga
A1 Seoane, Marcos
A1 Ferré, Susana
A1 Prieto, Jose M.
A1 Lema, Manuela
A1 Señarís Rodríguez, Rosa María
A1 Arce Vázquez, Víctor Manuel
K1 Astrocyte survival
K1 Interferon beta
K1 Multiple Sclerosis
AB Background: We have previously shown that interferon-β (IFN-β) is a potent promoter of astrocyte survival. Although the mechanism(s) by which IFN-β promotes astrocyte survival have not been completely elucidated, it has been shown that IFN-β directly stimulates survival signaling pathways. In the present report, we took advantage of the differences in the susceptibility of fetal and neonatal astrocytes to apoptosis to further investigate the mechanism(s) underlying the antiapoptotic effect of IFN-β. Methods: Primary monolayer cultures of cortical astrocytes were established from neonatal (3- to 6-day-old) or fetal (embryonic days: E15 or E17) Sprague-Dawley rat cerebral cortices. Apoptotic cell death was determined by fluorescent-microscopic analysis of staining patterns of cell DNA with Hoechst 33258, and determination of annexin V binding.Akt phosphorylation was detected by Western blottingusing a commercial kit that allows specific recognition of both non-phosphorylated and serine-phosphorylated Akt. Results: In the present work, we have found that primary astrocytes obtained from neonatal rats are resistant to apoptosis induced by serum starvation, though cell death may be induced by combining serum starvation with sodium butyrate treatment. This effect is counteracted by IFN-β treatment through a mechanism that involves phosphatidylinositol 3-kinase stimulation. Conclusions: IFN-β can be considered as a neuroprotective agent and, therefore, part of its beneficial effects in multiple sclerosis (MS) treatment may depend on its capacity to protect astrocytes against the apoptotic cell death that occurs in the course of the MS lesions.
PB Karger
SN 1021-7401
YR 2007
FD 2007-08-15
LK https://hdl.handle.net/10347/39044
UL https://hdl.handle.net/10347/39044
LA eng
NO Barca Mayo, O., Seoane, M., Ferré, S., Prieto, J.M., Lema, M., Señarís, R.M., Arce, V.M. (2007). Mechanisms of interferon-β-induced survival in fetal and neonatal primary astrocytes. “Neuroimmunomodulation”, vol. 14 (1), 39-45.
NO Fondo de Investigaciones Sanitarias (FIS 99/1188 and FIS 01/1007)
DS Minerva
RD 22 abr 2026