RT Journal Article
T1 Modeling Cancer Using Zebrafish Xenografts: Drawbacks for Mimicking the Human Microenvironment
A1 Cabezas Sáinz, Pablo
A1 Pensado López, Alba
A1 Sainz Anding, Bruno
A1 Sánchez Piñón, Laura
K1 Zebrafish
K1 Xenograft
K1 Cancer
K1 Temperature
K1 Microenvironment
K1 Chemotherapy
AB The first steps towards establishing xenografts in zebrafish embryos were performed by Lee et al., 2005 and Haldi et al., 2006, paving the way for studying human cancers using this animal species. Since then, the xenograft technique has been improved in different ways, ranging from optimizing the best temperature for xenografted embryo incubation, testing different sites for injection of human tumor cells, and even developing tools to study how the host interacts with the injected cells. Nonetheless, a standard protocol for performing xenografts has not been adopted across laboratories, and further research on the temperature, microenvironment of the tumor or the cell–host interactions inside of the embryo during xenografting is still needed. As a consequence, current non-uniform conditions could be affecting experimental results in terms of cell proliferation, invasion, or metastasis; or even overestimating the effects of some chemotherapeutic drugs on xenografted cells. In this review, we highlight and raise awareness regarding the different aspects of xenografting that need to be improved in order to mimic, in a more efficient way, the human tumor microenvironment, resulting in more robust and accurate in vivo results
PB MDPI
YR 2020
FD 2020
LK http://hdl.handle.net/10347/26110
UL http://hdl.handle.net/10347/26110
LA eng
NO Cells 2020, 9(9), 1978; https://doi.org/10.3390/cells9091978
NO Consellería de Educación, Universidade e Formación Profesional (ED431C 2018/28)
DS Minerva
RD 24 abr 2026