RT Journal Article T1 Green approaches for the extraction of antioxidants from eucalyptus leaves A1 Gullón Estévez, Beatriz A1 Muñiz Mouro, Abel A1 Lu Chau, Thelmo Alejandro A1 Moreira Vilar, María Teresa A1 Lema Rodicio, Juan Manuel A1 Eibes, A1 Eibes González, Gemma María AB Leaf extracts of Eucalyptus globulus present a wide range of phenolic compounds with interest in the pharmaceutical, health, agricultural, cosmetic and food industries because of their medicinal properties. Although conventional solvent extraction is the most extensively used methodology to extract and isolate these active compounds, the excessive consumption of time and energy makes its application relatively inefficient. This study investigates other more cost-effective and environmentally friendly techniques for the isolation of antioxidant phenolic compounds, such as enzyme assisted extraction (EAE), microwave assisted extraction (MAE), ultrasound assisted extraction (UAE), and deep eutectic solvent (DES) extraction. First, the conventional extraction was used to evaluate the effect of the particle size and the liquid-to-solid ratio. Next, relevant parameters of the green extraction techniques were optimized, such as time of extraction, enzyme and dose used, microwave power, and type of DES. The optimized extracts from MAE, UAE, DES and conventional extraction were characterized by UHPLC-ESI-MS, showing a similar phenolic profile with 26 tentatively identified compounds. Finally, the conditions leading to the extracts with the highest content in phenolics were evaluated in terms of specific energy consumption. The lowest specific energy consumption was obtained with MAE, with a value more than 13 times lower than conventional extraction. PB Elsevier YR 2019 FD 2019-10-05 LK https://hdl.handle.net/10347/39270 UL https://hdl.handle.net/10347/39270 LA eng NO Beatriz Gullón, Abel Muñiz-Mouro, Thelmo A. Lú-Chau, María Teresa Moreira, Juan M. Lema, Gemma Eibes, Green approaches for the extraction of antioxidants from eucalyptus leaves, Industrial Crops and Products, Volume 138, 2019, 111473, ISSN 0926-6690, https://doi.org/10.1016/j.indcrop.2019.111473. DS Minerva RD 30 abr 2026