RT Journal Article
T1 A chemical approach for the synthesis of the DNA-binding domain of the oncoprotein MYC
A1 Calo Lapido, Renata
A1 Penas Tallón, Cristina
A1 Jiménez Balsa, Adrián
A1 Vázquez Sentís, Marco Eugenio
A1 Mascareñas Cid, José Luis
AB We describe the first chemical synthesis of a functional mutant of the DNA binding domain of the oncoprotein MYC, using two alternative strategies which involve either one or two Native Chemical Ligations (NCLs). Both routes allowed the efficient synthesis of a miniprotein which is capable of heterodimerizing with MAX, and replicate the DNA binding of the native protein. The versatility of the reported synthetic approach enabled the straightforward preparation of MYC and Omomyc analogues, as well as fluorescently labeled derivatives
PB Royal Society of Chemistry
YR 2019
FD 2019
LK http://hdl.handle.net/10347/20792
UL http://hdl.handle.net/10347/20792
LA eng
NO Calo-Lapido, R., Penas, C., Jiménez-Balsa, A., Vázquez, M. E., Mascareñas, J. L. (2019), A chemical approach for the synthesis of the DNA-binding domain of the oncoprotein MYC. Org. Biomol. Chem., 17: 6748-6752. doi:10.1039/C9OB01209E
NO This is the peer reviewed version of the following article: Calo-Lapido, R., Penas, C., Jiménez-Balsa, A., Vázquez, M. E., Mascareñas, J. L. (2019), A chemical approach for the synthesis of the DNA-binding domain of the oncoprotein MYC. Org. Biomol. Chem., 17: 6748-6752, which has been published in final form at https://doi.org/10.1039/C9OB01209E. This article may be used for non-commercial purposes in accordance with RSC Terms and Conditions for Use of Self-Archived Versions
NO Financial support from the MINECO (CTQ2015-70698-R and SAF2016-76689-R, orfeo-cinqa CTQ2016-81797-REDC), Xunta de Galicia (2015-CP082, ED431C 2017/19, ED431B 2018/04, Centro singular de investigación de Galicia accreditation 2016–2019, ED431G/09), the European Union (European Regional Development Fund - ERDF), Fundación AECC (IDEAS197VAZQ), and the European Research Council (Advanced Grant No. 340055) is acknowledged. R. C. L. thanks Xunta de Galicia and the European Social Fund for her fellowship. A. J. B. thanks Xunta de Galicia for his post-doctoral fellowship
DS Minerva
RD 4 may 2026