RT Journal Article
T1 Whole genome sequencing, molecular typing and in vivo virulence of OXA-48-producing Escherichia coli isolates including ST131 H30-Rx, H22 and H41 subclones
A1 Toro, María de
A1 Fernández, J.
A1 García, Vanesa
A1 Mora Gutiérrez, Azucena
A1 Blanco Álvarez, Jorge
A1 Cruz, Fernando de la
A1 Rodicio Rodicio, María del Rosario
K1 Virulence
K1 Carbapenemase
K1 Klebsiella
K1 Escherichia coli
AB Carbapenem-resistant Enterobacteriaceae, including the increasingly reported OXA-48 Escherichia coli producers, are an emerging public health threat worldwide. Due to their alarming detection in our healthcare setting and their possible presence in the community, seven OXA-48-producing, extraintestinal pathogenic E. coli were analysed by whole genome sequencing as well as conventional tools, and tested for in vivo virulence. As a result, five E. coli OXA-48-producing subclones were detected (O25:H4-ST131/PST43-fimH30-virotype E; O25:H4-ST131/PST9-fimH22-virotype D5, O16:H5-ST131/PST506-fimH41; O25:H5-ST83/PST207 and O9:H25-ST58/PST24). Four ST131 and one ST83 isolates satisfied the ExPEC status, and all except the O16:H5 ST131 isolate were UPEC. All isolates exhibited local inflammatory response with extensive subcutaneous necrosis but low lethality when tested in a mouse sepsis model. The bla OXA-48 gene was located in MOBP131/IncL plasmids (four isolates) or within the chromosome (three ST131 H30-Rx isolates), carried by Tn1999-like elements. All, except the ST83 isolate, were multidrug-resistant, with additional plasmids acting as vehicles for the spread of various resistance genes. This is the first study to analyse the whole genome sequences of bla OXA-48-positive ST131, ST58 and ST83 E. coli isolates in conjunction with experimental data, and to evaluate the in vivo virulence of bla OXA-48 isolates, which pose an important challenge to patient management
PB Springer Nature
SN 2045-2322
YR 2017
FD 2017
LK http://hdl.handle.net/10347/17617
UL http://hdl.handle.net/10347/17617
LA eng
NO de Toro, M., Fernández, J., García, V. et al. Whole genome sequencing, molecular typing and in vivo virulence of OXA-48-producing Escherichia coli isolates including ST131 H30-Rx, H22 and H41 subclones. Sci Rep 7, 12103 (2017). https://doi.org/10.1038/s41598-017-12015-0
NO Work at USC-LREC was supported by projects AGL2013–47852-R from the Ministerio de Economía y Competitividad (MINECO, Spain) and Fondo Europeo de Desarrollo Regional (FEDER); AGL2016–79343-R from the Agencia Estatal de Investigación (AEI, Spain) and FEDER; PI16/01477 from Plan Estatal de I + D + I 2013–2016, Instituto de Salud Carlos III (ISCIII), Subdirección General de Evaluación y Fomento de la Investigación, and FEDER; CN2012/303 from the Consellería de Cultura, Educación e Ordenación Universitaria, (Xunta de Galicia) and FEDER. Work at the FdlC laboratory was financed by the Spanish Ministry of Economy and Competitiveness (projects BFU2014–55534-C2-1-P and RTC-2015-3184-1). Work at the UO was supported by projects FIS PI11-00808 (Fondo de Investigación Sanitaria, ISCIII, Ministerio de Economía y Competitividad, Spain) co-funded by FEDER and UO-15-INVES-09 (Consejería de Educación, Cultura y Deporte del Principado de Asturias, Spain). A. Mora acknowledges the Ministerio de Educación, Cultura y Deporte (Spain) for the mobility grant for teachers and researchers from the Programa Estatal de Promoción del Talento y su Empleabilidad, Plan Estatal de Investigación Científica y Técnica y de Innovación 2013–2016
DS Minerva
RD 30 abr 2026