RT Journal Article
T1 Steps of the Replication Cycle of the Viral Haemorrhagic Septicaemia Virus (VHSV) Affecting Its Virulence on Fish
A1 López Vázquez, Carmen
A1 Bandín Matos, Isabel
A1 Panzarin, Valentina
A1 Toffan, Anna
A1 Cuenca, Argelia
A1 Olesen, Niels J.
A1 Pereira Dopazo, Carlos
K1 Novirhabdovirus
K1 Viral cycle
K1 Viral production
K1 RNA synthesis
K1 Trout
AB The viral haemorrhagic septicaemia virus (VHSV), a single-stranded negative-sense RNA novirhabdovirus affecting a wide range of marine and freshwater fish species, is a main concern for European rainbow trout (Oncorhynchus mykiss) fish farmers. Its genome is constituted by six genes, codifying five structural and one nonstructural proteins. Many studies have been carried out to determine the participation of each gene in the VHSV virulence, most of them based on genome sequence analysis and/or reverse genetics to construct specific mutants and to evaluate their virulence phenotype. In the present study, we have used a different approach with a similar aim: hypothesizing that a failure in any step of the replication cycle can reduce the virulence in vivo, we studied in depth the in vitro replication of VHSV in different cell lines, using sets of strains from different origins, with high, low and moderate levels of virulence for fish. The results demonstrated that several steps in the viral replication cycle could affect VHSV virulence in fish, including adsorption, RNA synthesis and morphogenesis (including viral release). Notably, differences among strains in any step of the replication cycle were mostly strain-specific and reflected only in part the in vivo phenotype (high and low virulent). Our data, therefore, support the need for further studies aimed to construct completely avirulent VHSV recombinants targeting a combination of genes rather than a single one in order to study the mechanisms of genes interplay and their effect on viral phenotype in vitro and in vivo
PB MDPI
YR 2020
FD 2020
LK http://hdl.handle.net/10347/24024
UL http://hdl.handle.net/10347/24024
LA eng
NO López-Vázquez, C.; Bandín, I.; Panzarin, V.; Toffan, A.; Cuenca, A.; Olesen, N.J.; Dopazo, C.P. Steps of the Replication Cycle of the Viral Haemorrhagic Septicaemia Virus (VHSV) Affecting Its Virulence on Fish. Animals 2020, 10, 2264
NO The project has been funded under the ERANET. The content of this article reflects only the authors’ views, and the ERANET Consortium is not liable for any use that may be made of the information contained therein
DS Minerva
RD 27 abr 2026