RT Journal Article
T1 A single genomic region involving a putative chromosome rearrangement in flat oyster (Ostrea edulis) is associated with differential host resilience to the parasite Bonamia ostreae
A1 Martínez Sambade, Inés
A1 Casanova Chiclana, Adrián
A1 Blanco Hortas, Andrés
A1 Gundappa, Manu K.
A1 Bean, Tim P.
A1 Macqueen, Daniel J.
A1 Houston, Ross D.
A1 Villalba García, Antonio
A1 Vera Rodríguez, Manuel
A1 Kamermans, Pauline
A1 Martínez Portela, Paulino
K1 Bonamiosis
K1 Chromosome rearrangement
K1 Disease resilience
K1 Ostrea edulis
K1 SNP-chip
AB European flat oyster (Ostrea edulis) is an ecologically and economically important marine bivalve, that has been severely affected by the intracellular parasite Bonamia ostreae. In this study, a flat oyster SNP array (~14,000 SNPs) was used to validate previously reported outlier loci for divergent selection associated with B. ostreae exposure in the Northeast Atlantic Area. A total of 134 wild and hatchery individuals from the North Sea, collected in naïve (NV) and long-term affected (LTA) areas, were analysed. Genetic diversity and differentiation were related to the sampling origin (wild vs. hatchery) when using neutral markers, and to bonamiosis status (NV vs. LTA) when using outlier loci for divergent selection. Two genetic clusters appeared intermingled in all sampling locations when using outlier loci, and their frequency was associated with their bonamiosis status. When both clusters were compared, outlier data sets showed high genetic divergence (FST > 0.25) unlike neutral loci (FST not ≠ 0). Moreover, the cluster associated with LTA samples showed much higher genetic diversity and significant heterozygote excess with outlier loci, but not with neutral data. Most outliers mapped on chromosome 8 (OE-C8) of the flat oyster genome, supporting a main genomic region underlying resilience to bonamiosis. Furthermore, differentially expressed genes previously reported between NV and LTA strains showed higher mapping density on OE-C8. A range of relevant immune functions were specifically enriched among genes annotated on OE-C8, providing hypotheses for resilience mechanisms to an intracellular parasite. The results suggest that marker-assisted selection could be applied to breed resilient strains of O. edulis to bonamiosis, if lower parasite load and/or higher viability of the LTA genetic cluster following B. ostreae infection is demonstrated
PB Wiley
SN 1752-4571
YR 2022
FD 2022
LK http://hdl.handle.net/10347/30199
UL http://hdl.handle.net/10347/30199
LA eng
NO Manu Kumar Gundappa, Carolina Peñaloza, Tim Regan, Isabelle Boutet, Arnaud Tanguy, Ross D. Houston, Tim P. Bean, Daniel J. Macqueen, Chromosome‐level reference genome for European flat oyster (Ostrea edulis L.), Evolutionary Applications, 10.1111/eva.13460, 15, 11, (1713-1729)
NO Biotechnology and Biological Sciences Research Council. Grant Numbers: BBS/E/D/20002172, BB/S004181/1. Ministry of Agriculture, Nature and Food safety. Grant Numbers: BO43-18, KB33-004
DS Minerva
RD 28 abr 2026