RT Journal Article
T1 Supramolecular Recognition and Selective Protein Uptake by Peptide Hybrids
A1 Juanes Carrasco, Marisa
A1 Lostalé Seijo, Irene
A1 Granja Guillán, Juan Ramón
A1 Montenegro García, Javier
K1 Supramolecular systems
K1 Membranes
K1 Peptides
K1 Penetrating peptides
K1 Amphiphiles
K1 Protein
K1 Transport
AB The intracellular transport of exogenous proteins has emerged as one of the most promising methodologies for biotechnology and chemical biology. Current protein delivery is mainly approached by liposome encapsulation, translational fusion and ionic/hydrophobic non‐covalent aggregation with transporting molecular vehicles. We here introduce the concept of supramolecular recognition and selective transport of proteins by peptide hybrid materials. We have designed a helical amphiphilic cationic peptide that bears two orthogonal alkoxyamines for the precise anchoring of protein ligands. After the attachment of these protein ligands, the peptide showed a high binding affinity for its protein target (i.e. mannose/Concanavalin A, Biotin/Streptavidin). The resulting peptide/protein hybrids were taken up by human cells such as HeLa and HepG2. The concept described in this manuscript could potentially be adapted, through the appropriate choice of ligands, to the transport of other proteins with suitable supramolecular binding motifs
PB Wiley
YR 2018
FD 2018-04-27
LK http://hdl.handle.net/10347/16690
UL http://hdl.handle.net/10347/16690
LA eng
NO Juanes, M., Lostalé-Seijo, I., Granja, J., & Montenegro, J. (2018). Supramolecular Recognition and Selective Protein Uptake by Peptide Hybrids. Chemistry - A European Journal. http://dx.doi.org/10.1002/chem.201800706
NO This manuscript has been accepted after peer review and appears as anAccepted Article online prior to editing, proofing, and formal publicationof the final Version of Record (VoR). This work is currently citable byusing the Digital Object Identifier (DOI) given below. The VoR will bepublished online in Early View as soon as possible and may be differentto this Accepted Article as a result of editing. Readers should obtainthe  VoR  from  the  journal  website  shown  below  when  it  is  publishedto ensure accuracy of information. The authors are responsible for thecontent of this Accepted Article
NO This work was partially supported by the Spanish Agencia Estatal de Investigación (AEI) [CTQ2014-59646-R, CTQ2013-43264-R, SAF2017-89890-R], the Xunta de Galicia (ED431G/09, ED431C 2017/25 and 2016-AD031) and the ERDF. We thank Pili Canoa (CACTI, UVIGO) for assistance with the SPR measurements. M.J. received a F.P.I. fellowship from MINECO (CTQ2014-59646-R). J.M. received a Ramón y Cajal (RYC-2013-13784), an ERC Starting Investigator Grant (DYNAP- 677786) and a Young Investigator Grant from the Human Frontier Science Research Program (RGY0066/2017)
DS Minerva
RD 28 abr 2026