RT Journal Article
T1 Soil bacterial community tolerance to three tetracycline antibiotics induced by Ni and Zn
A1 Santás Miguel, Vanesa
A1 Rodríguez González, Laura
A1 Núñez Delgado, Avelino
A1 Álvarez Rodríguez, Esperanza
A1 Díaz-Raviña, Montserrat
A1 Arias Estévez, Manuel
A1 Fernández Calviño, David
K1 Antibiotics
K1 Bacterial growth
K1 Heavy metals
K1 Leucine incorporation method
K1 PICT
AB A laboratory work has been carried out to determine the tolerance of soil bacterialcommunities to Ni and Zn and co-tolerance to tetracycline antibiotics (chlortetracycline(CTC), oxytetracycline (OTC) and tetracycline (TC)) in soils individually spiked with fivedifferent concentrations of Ni or Zn (1,000, 750, 500, 250, and 125 mg kg−1), and anuncontaminated (0 mg kg−1) control soil. The PICT parameter (pollution-inducedcommunity tolerance) was estimated for the bacterial community using the tritium (3H)-labeled leucine incorporation technique, and the values corresponding to log IC50 wereused as toxicity index. The mean log IC50 values observed in the uncontaminated soilsamples indicate that Zn (with log IC50 = −2.83) was more toxic than Ni (log IC50 = −2.73).In addition, for the soil with the lowest carbon content (C = 1.9%), Ni-contaminatedsamples showed increased tolerance when the Ni concentrations addedwere ≥500 mg kg−1, while for the soils with higher carbon content (between 5.3% and10.9%) tolerance increased when Ni concentrations added were ≥1,000 mg kg−1.Regarding the soils contaminated with Zn, tolerance increased in all the soils studiedwhen the Zn concentrations added were ≥125 mg kg−1, regardless of the soil carboncontent. The co-tolerance increases obtained after exposure of the bacterial suspension toTC, OTC and CTC showed an identical behavior within these tetracycline antibiotics.However, it was dependent on the heavy metal tested (Ni or Zn). In the case of soils 1 (C =1.1%) and 2 (C = 5.3%), the soil bacterial communities showed increases in co-toleranceto TC, OTC and CTC for Ni concentrations added of ≥125 mg kg−1, while for soil 3 (withC = 10.9%) co-tolerance took place when Ni was added at ≥1,000 mg kg−1. However, insoils contaminated with Zn, increases in co-tolerance to CTC, OTC and TC occurred at Znconcentrations added of ≥125 mg kg−1 for the 3 soils tested. These results can beconsidered relevant when anticipating possible environmental repercussions related tothe simultaneous presence of various types of pollutants, specifically certain heavy metalsand antibiotics.
PB Frontiers Media
YR 2023
FD 2023
LK http://hdl.handle.net/10347/34046
UL http://hdl.handle.net/10347/34046
LA eng
NO Santás-Miguel, V., Rodríguez-González, L., Núñez-Delgado, A., Álvarez-Rodríguez, E., Díaz-Raviña, M., Arias-Estévez, M. & Fernández-Calviño, D. (2023). Soil bacterial community tolerance to three tetracycline antibiotics induced by Ni and Zn. Spanish Journal of Soil Science, 13. https://doi.org/10.3389/sjss.2023.10799
NO This study has been funded by the Spanish Ministry of Economyand Competitiveness through the projects CGL 2015-67333-C2-1-R and -2-R (FEDER Funds) and by Xunta de Galicia viaBV1 research group (ED431C 2017/62-GRC). F-CD holds aRamón y Cajal contract (RYC-2016-20411) financed by theSpanish Ministry of Economy, Industry and Competitiveness.S-MV Santás Miguel holds a posdoctoral fellowship (ED481B2022-081) financed by Xunta de Galicia. A-RL hold a predoctoral fellowship (ED481A 2021/309) financed by Xunta deGalicia.
DS Minerva
RD 29 abr 2026