RT Journal Article
T1 The ST131 Escherichia coli H22 subclone from human intestinal microbiota: Comparison of genomic and phenotypic traits with those of the globally successful H30 subclone
A1 Nicolas Chanoine, Marie Hélène
A1 Petitjean, Marie
A1 Mora Gutiérrez, Azucena
A1 Mayer, Noémie
A1 Lavigne, Jean-Philippe
A1 Boulet, Olivier
A1 Leflon-Guibout, Veronique
A1 Blanco Álvarez, Jorge
A1 Hocquet, Didier
K1 E. coli ST131
K1 H22 genome
K1 Sugar metabolism
K1 Mannose-binding FimH region
K1 Biofilm
K1 Subclades B
K1 Plasmid replicons
AB In 2006, we found healthy subjects carrying ST131 Escherichia coli in their intestinal microbiota consisting of two populations: a subdominant population of fluoroquinolone-resistant E. coli belonging to subclone H30 (H30-R or subclade C1), the current worldwide dominant ST131 subclone, and a dominant E. coli population composed of antibiotic-susceptible E. coli belonging to subclone H22 (clade B), the precursor of subclone H30. Wesequenced the whole genome of fecal H22 strain S250, compared it to the genomes of ExPEC ST131 H30-Rx strain JJ1886 and commensal ST131 H41 strain SE15, sought the H22-H30 genomic differences in our fecal strains and assessed their phenotypic consequences. We detected 173 genes found in the Virulence Factor Database, of which 148 were shared by the three ST131 genomes, whereas some were genome-specific, notably those allowing determination of virotype (D for S250 and C for JJ1886). We found three sequences of the FimH site involved in adhesion: two in S250 and SE15 close and identical, respectively, to that previously reported to confer strong intestinal adhesion, and one in JJ1886, corresponding to that commonly present in uropathogenic E. coli. Among the genes involved in sugar metabolism, one encoding a gluconate kinase lacked in S250 and JJ1886. Although this gene was also absent in both our fecal H22 and H30-R strains, H22 strains showed a higher capacity to grow in minimal medium with gluconate. Among the genes involved in gluconate metabolism, only the ghrB gene differed between S250/H22 and JJ1886/H30-R strains, resulting in different gluconate reductases. Of the genes involved in biofilm formation, two were absent in the three genomes and one, fimB, in the JJ1886 genome. Our fecal H30-R strains lacking intact fimB displayed delayed biofilm formation relative to our fecal H22 strains. The H22 strains differed by subclade B type and plasmidcontent, whereas the H30-R strains were identical. Phenotypic analysis of our fecal strains based on observed genomic differences between S250 and JJ1886 strains suggests the presence of traits related to bacterial commensalism in our H22 strains and traits commonly found in uropathogenic E. coli in our H30-R strains.
PB Biomed Central
SN 1471-2180
YR 2017
FD 2017-03
LK http://hdl.handle.net/10347/15937
UL http://hdl.handle.net/10347/15937
LA eng
NO Nicolas-Chanoine, M. H., Petitjean, M., Mora, A., Mayer, N., Lavigne, J. P., Boulet, O., ... & Hocquet, D. (2017). The ST131 Escherichia coli H 22 subclone from human intestinal microbiota: Comparison of genomic and phenotypic traits with those of the globally successful H 30 subclone. BMC microbiology, 17(1), 71.
NO This work was partially supported by grants AGL2013-47852-R (Ministerio de Economía y Competitividad, Gobierno de España), CN2012/303 and EM2014/001 (Consellería de Cultura, Educación e Ordenación Universitaria, Xunta de Galicia and The European Regional Development Fund, ERDF), and RCT-2012-02 (Direction Générale de l’Offre de Soins et Institut National de la Santé et de la Recherche Médicale, Paris, France)
DS Minerva
RD 29 abr 2026