RT Journal Article
T1 Preparation of functionalized magnetic nanoparticles conjugated with feroxamine and their evaluation for pathogen detection
A1 Martínez Matamoros, Diana
A1 Castro García, Socorro
A1 Balado Dacosta, Miguel
A1 Matamoros Veloza, Adriana
A1 Camargo Valero, Miller Alonso
A1 Cespedes, Oscar
A1 Rodríguez, Jaime
A1 Jiménez, Carlos
A1 Lemos Ramos, Manuel Luis
K1 Nanoparticles
K1 Feroxamine
K1 Pathogen
AB This work reports the preparation of a conjugate between amino-functionalized silica magnetite and thesiderophore feroxamine. The morphology and properties of the conjugate and intermediate magneticnanoparticles (MNPs) were examined by powder X-ray diffraction (XRD), Fourier Transform Infraredspectroscopy (FT-IR), Raman spectroscopy, X-ray photoelectron spectroscopy (XPS), magnetizationstudies, zeta potential measurements, Transmission Electron Microscopy (TEM) and Energy Dispersive X-ray (EDX) mapping. Furthermore, this study investigated the interaction between the functionalizedmagnetic NPs andYersinia  enterocoliticawild type (WC-A) using Scanning Electron Microscopy (SEM)and TEM images. In addition, the interaction between MNPs and aY. enterocoliticamutant strain lackingferoxamine receptor FoxA, was also used to study the binding specificity. The results showed that thecapture and isolation ofY.  enterocoliticaby the MNPs took place in all cases. Moreover, the specificinteraction between the MNP conjugate and bacteria did not increase after blocking the free aminegroups witht-butoxycarbonyl (Boc) and carboxylic acid (COOH) functional groups. Electrostatic surfaceinteractions instead of molecular recognition between MNP conjugate and feroxamine receptor seem torule the attachment of bacteria to the conjugate
PB Royal Society of Chemistry
SN 2046-2069
YR 2019
FD 2019
LK http://hdl.handle.net/10347/23119
UL http://hdl.handle.net/10347/23119
LA eng
NO Martínez Matamoros, D., Castro García, S., Balado Dacosta, M., Matamoros Veloza, A., Camargo Valero, M.A., Cespedes, O. et al. (2019). Preparation of functionalized magnetic nanoparticles conjugated with feroxamine and their evaluation for pathogen detection. RSC Advances, vol. 9, 13533-13542
NO This work was supported by grants AGL2015-63740-C2-2-R and AGL2015-63740-C2-1-R (AEI/FEDER, EU) from the State Agency for Research (AEI) of Spain, both co-funded by the FEDER Programme from the European Union
DS Minerva
RD 3 may 2026