RT Journal Article
T1 Sterile and dual-porous aerogels scaffolds obtained through a multistep supercritical CO2-based approach
A1 Santos Rosales, Víctor
A1 Ardao Palacios, Inés
A1 Álvarez Lorenzo, Carmen
A1 Ribeiro, Nilza
A1 Oliveira, Ana L.
A1 García González, Carlos A.
K1 Starch aerogels
K1 Zein
K1 Supercritical sterilization
K1 Supercritical CO2
K1 Regenerative medicine
AB Aerogels from natural polymers are endowed with attractive textural and biological properties for biomedical applications due to their high open mesoporosity, low density, and reduced toxicity. Nevertheless, the lack of macroporosity in the aerogel structure and of a sterilization method suitable for these materials restrict their use for regenerative medicine purposes and prompt the research on getting ready-to-implant dual (macro + meso)porous aerogels. In this work, zein, a family of proteins present in materials for tissue engineering, was evaluated as a sacrificial porogen to obtain macroporous starch aerogels. This approach was particularly advantageous since it could be integrated in the conventional aerogel processing method without extra leaching steps. Physicochemical, morphological, and mechanical characterization were performed to study the effect of porogen zein at various proportions (0:1, 1:2, and 1:1 zein:starch weight ratio) on the properties of the obtained starch-based aerogels. From a forward-looking perspective for its clinical application, a supercritical CO2 sterilization treatment was implemented for these aerogels. The sterilization efficacy and the influence of the treatment on the aerogel final properties were evaluated mainly in terms of absence of microbial growth, cytocompatibility, as well as physicochemical, structural, and mechanical modifications.
PB MDPI
YR 2019
FD 2019
LK http://hdl.handle.net/10347/21327
UL http://hdl.handle.net/10347/21327
LA eng
NO Santos-Rosales, V.; Ardao, I.; Alvarez-Lorenzo, C.; Ribeiro, N.; Oliveira, A.L.; García-González, C.A. Sterile and Dual-Porous Aerogels Scaffolds Obtained through a Multistep Supercritical CO2-Based Approach. Molecules 2019, 24, 871
NO Work supported by Xunta de Galicia [ED431F 2016/010 & ED431C 2016/008], MINECO [SAF2017-83118R], AEI, FEDER and Interreg VAPOCTEP Programme [0245_IBEROS_1_E].C.A.García-González acknowledges to MINECO for a Ramón y Cajal Fellowship [RYC2014-15239]. V. Santos-Rosales acknowledges to Xunta de Galicia (Consellería de Cultura, Educación e Ordenación Universitaria) for a predoctoral research fellowship [ED481A-2018/014]. A.L. Oliveira acknowledges Portuguese National Funds from FCT—Fundação para a Ciência e a Tecnologia through Program FCT Investigator (IF/00411/2013), project SERICAMED (IF/00411/2013/CP1167) and project UID/Multi/50016/2013. Work carried out in the frame of the COST-Action “Advanced Engineering and Research of aeroGels for Environment and Life Sciences” (AERoGELS, ref. CA18125) funded by the European Commission
DS Minerva
RD 25 abr 2026