RT Journal Article
T1 Revisiting the Taxonomy of the Genus Arcobacter: Getting Order From the Chaos
A1 Pérez Cataluña, Alba
A1 Salas Massó, Nuria
A1 López Diéguez, Ana Belén
A1 Balboa Méndez, Sabela
A1 Lema Blanco, Alberto
A1 López Romalde, Jesús
A1 Figueras, Maria J.
K1 Arcobacter
K1 Aliiarcobacter gen. nov.
K1 Pseudoarcobacter gen. nov.
K1 Haloarcobacter gen. nov.
K1 Poseidonibacter gen. nov.
K1 Malacobacter gen. nov.
K1 Taxonomic criteria
AB Since the description of the genus Arcobacter in 1991, a total of 27 species have been described, although some species have shown 16S rRNA similarities below 95%, which is the cut-off that usually separates species that belong to different genera. The objective of the present study was to reassess the taxonomy of the genus Arcobacter using information derived from the core genome (286 genes), a Multilocus Sequence Analysis (MLSA) with 13 housekeeping genes, as well as different genomic indexes like Average Nucleotide Identity (ANI), in silico DNA–DNA hybridization (isDDH), Average Amino-acid Identity (AAI), Percentage of Conserved Proteins (POCPs), and Relative Synonymous Codon Usage (RSCU). The study included a total of 39 strains that represent all the 27 species included in the genus Arcobacter together with 13 strains that are potentially new species, and the analysis of 57 genomes. The different phylogenetic analyses showed that the Arcobacter species grouped into four clusters. In addition, A. lekithochrous and the candidatus species ‘A. aquaticus’ appeared, as did A. nitrofigilis, the type species of the genus, in separate branches. Furthermore, the genomic indices ANI and isDDH not only confirmed that all the species were well-defined, but also the coherence of the clusters. The AAI and POCP values showed intra-cluster ranges above the respective cut-off values of 60% and 50% described for species belonging to the same genus. Phenotypic analysis showed that certain test combinations could allow the differentiation of the four clusters and the three orphan species established by the phylogenetic and genomic analyses. The origin of the strains showed that each of the clusters embraced species recovered from a common or related environment. The results obtained enable the division of the current genus Arcobacter in at least seven different genera, for which the names Arcobacter, Aliiarcobacter gen. nov., Pseudoarcobacter gen. nov., Haloarcobacter gen. nov., Malacobacter gen. nov., Poseidonibacter gen. nov., and Candidate ‘Arcomarinus’ gen. nov. are proposed
PB Frontiers Media
YR 2018
FD 2018-09-04
LK http://hdl.handle.net/10347/17649
UL http://hdl.handle.net/10347/17649
LA eng
NO Pérez-Cataluña A, Salas-Massó N, Diéguez AL, Balboa S, Lema A, Romalde JL and Figueras MJ (2018) Revisiting the Taxonomy of the Genus Arcobacter: Getting Order From the Chaos. Front. Microbiol. 9:2077. doi: 10.3389/fmicb.2018.02077
NO This work was supported in part by Grants JPIW2013-69095-C03-03 from the Ministerio de Economía y Competitividad (MINECO), AQUAVALENS of the Seventh Framework Program (FP7/2007-2013) grant agreement 311846 from the European Union and AGL2013-42628-R and AGL2016-77539-R (AEI/FEDER UE) from the Agencia Estatal de Investigación (Spain)
DS Minerva
RD 24 abr 2026