RT Journal Article
T1 Experimental evidence of harmful effects of Crithidia mellificae and Lotmaria passim on honey bees
A1 Gómez Moracho, Tamara
A1 Buendía Abad, María
A1 Benito Murcia, María
A1 García Palencia, Pilar
A1 Barrios, Laura
A1 Bartolomé Husson, Carolina
A1 Maside Rodríguez, Xulio Manuel
A1 Meana Mañes, María Aránzazu
A1 Jiménez Antón, María Dolores
A1 Olías Molero, Ana Isabel
A1 Alunda Rodríguez, José María
A1 Martín Hernández, Raquel
A1 Higes Pascual, Mariano
K1 Lotmaria passim
K1 Crithidia mellificae
K1 Culture media
K1 Morphotypes
K1 Experimental infection
K1 Honey bee survival
AB The trypanosomatids Crithidia mellificae and Lotmaria passim are very prevalent in honey bee colonies and potentially contribute to colony losses that currently represent a serious threat to honey bees. However, potential pathogenicity of these trypanosomatids remains unclear and since studies of infection are scarce, there is little information about the virulence of their different morphotypes. Hence, we first cultured C. mellificae and L. passim (ATCC reference strains) in six different culture media to analyse their growth rates and to obtain potentially infective morphotypes. Both C. mellificae and L. passim grew in five of the media tested, with the exception of M199. These trypanosomatids multiplied fastest in BHI medium, in which they reached a stationary phase after around 96 h of growth. Honey bees inoculated with either Crithidia or Lotmaria died faster than control bees and their mortality was highest when they were inoculated with 96 h cultured L. passim. Histological and Electron Microscopy analyses revealed flagellated morphotypes of Crithidia and Lotmaria in the lumen of the ileum, and adherent non-flagellated L. passim morphotypes covering the epithelium, although no lesions were evident. These data indicate that parasitic forms of these trypanosomatids obtained from the early stationary growth phase infect honey bees. Therefore, efficient infection can be achieved to study their intra-host development and to assess the potential pathogenicity of these trypanosomatids
PB Elsevier
SN 0020-7519
YR 2020
FD 2020-08-18
LK https://hdl.handle.net/10347/38541
UL https://hdl.handle.net/10347/38541
LA eng
NO Tamara Gómez-Moracho, María Buendía-Abad, María Benito, Pilar García-Palencia, Laura Barrios, Carolina Bartolomé, Xulio Maside, Aránzazu Meana, María Dolores Jiménez-Antón, Ana Isabel Olías-Molero, José María Alunda, Raquel Martín-Hernández, Mariano Higes, Experimental evidence of harmful effects of Crithidia mellificae and Lotmaria passim on honey bees, International Journal for Parasitology, Volume 50, Issue 13, 2020, Pages 1117-1124, ISSN 0020-7519, https://doi.org/10.1016/j.ijpara.2020.06.009
DS Minerva
RD 24 abr 2026