RT Journal Article
T1 Protein delivery based on uncoated and chitosan-coated mesoporous silicon microparticles
A1 Pastor, Ester L.
A1 Matveeva, Eugenia
A1 Valle Gallego, Ángela
A1 Goycoolea, Francisco M.
A1 García Fuentes, Marcos
K1 Porous silicon
K1 Electrochemical pore formation
K1 Chitosan
K1 Insulin
K1 BSA
K1 Protein
AB Mesoporous silicon is a biocompatible, biodegradable material that is receiving increased attention for pharmaceutical applications due to its extensive specific surface. This feature enables to load a variety of drugs in mesoporous silicon devices by simple adsorption-based procedures. In this work, we have addressed the fabrication and characterization of two new mesoporous silicon devices prepared by electrochemistry and intended for protein delivery, namely: (i) mesoporous silicon microparticles and (ii) chitosan-coated mesoporous silicon microparticles. Both carriers were investigated for their capacity to load a therapeutic protein (insulin) and a model antigen (bovine serum albumin) by adsorption. Our results show that mesoporous silicon microparticles prepared by electrochemical methods present moderate affinity for insulin and high affinity for albumin. However, mesoporous silicon presents an extensive capacity to load both proteins, leading to systems were protein could represent the major mass fraction of the formulation. The possibility to form a chitosan coating on the microparticles surface was confirmed both qualitatively by atomic force microscopy and quantitatively by a colorimetric method. Mesoporous silicon microparticles with mean pore size of 35 nm released the loaded insulin quickly, but not instantaneously. This profile could be slowed to a certain extent by the chitosan coating modification. With their high protein loading, their capacity to provide a controlled release of insulin over a period of 60-90 min, and the potential mucoadhesive effect of the chitosan coating, these composite devices comprise several features that render them interesting candidates as transmucosal protein delivery systems.
PB Elsevier
SN 1873-4367
YR 2011
FD 2011
LK http://hdl.handle.net/10347/9864
UL http://hdl.handle.net/10347/9864
LA eng
NO Pastor E., Matveeva E., Valle-Gallego A., Goycoolea F.M. and Garcia-Fuentes M. (2011) Protein delivery based on uncoated and chitosan-coated mesoporous silicon microparticles. Colloid Surface B, 88, 601-609
DS Minerva
RD 28 abr 2026