RT Journal Article
T1 Presence does not imply activity: DNA and RNA patterns differ in response to salt perturbation in anaerobic digestion
A1 De Vrieze, Jo
A1 Regueiro Abelleira, Leticia
A1 Props, Ruben
A1 Vilchez Vargas, Ramiro
A1 Járegui, Ruy
A1 Pieper, Dietmar H.
A1 Lema Rodicio, Juan Manuel
A1 Carballa Arcos, Marta
K1 Archae
K1 Biogas
K1 Illumina sequencing
K1 Methanogenesis
K1 Salinity
AB BackgroundThe microbial community in anaerobic digestion is mainly monitored by means of DNA-based methods. This may lead to incorrect interpretation of the community parameters, because microbial abundance does not necessarily reflect activity. In this research, the difference between microbial community response on DNA (total community) and RNA (active community) based on the 16S rRNA (gene) with respect to salt concentration and response time was evaluated.ResultsThe application of higher NaCl concentrations resulted in a decrease in methane production. A stronger and faster response to salt concentration was observed on RNA level. This was reflected in terms of microbial community composition and organization, as richness, evenness, and overall diversity were differentially impacted. A higher divergence of community structure was observed on RNA level as well, indicating that total community composition depends on deterministic processes, while the active community is determined by stochastic processes. Methanosaeta was identified as the most abundant methanogen on DNA level, but its relative abundance decreased on RNA level, related to salt perturbation.ConclusionsThis research demonstrated the need for RNA-based community screening to obtain reliable information on actual community parameters and to identify key species that determine process stability
PB BioMed Central
SN 1754-6834
YR 2016
FD 2016-10-20
LK http://hdl.handle.net/10347/15287
UL http://hdl.handle.net/10347/15287
LA eng
NO De Vrieze, J., Regueiro, L., Props, R., Vilchez-Vargas, R., Jáuregui, R., Pieper, D., Lema, J. and Carballa, M. (2016). Presence does not imply activity: DNA and RNA patterns differ in response to salt perturbation in anaerobic digestion. Biotechnology for Biofuels, 9(1)
NO This research was supported by the Spanish Ministry of Economy and Competitiveness and COMPLETE BELGIUM through REWATER project (EU ERANET NEWINDIGO—DST. PRI-PIMNIN-2011-1487). Jo De Vrieze is supported as postdoctoral fellow from the Research Foundation Flanders (FWO-Vlaanderen). Ruben Props is supported by Ghent University (BOFDOC2015000601) and the Belgian Nuclear Research Centre (SCK CEN). Leticia Regueiro, Juan M. Lema, and Marta Carballa belong to CRETUS (AGRUP2015/02) and to the Galician Competitive Research Group (GRC 2013-032)
DS Minerva
RD 28 abr 2026