RT Journal Article
T1 Ruthenium-Catalyzed Azide–Thioalkyne Cycloadditions in Aqueous Media: A Mild, Orthogonal, and Biocompatible Chemical Ligation
A1 Destito, Paolo
A1 Rodríguez Couceiro, José
A1 Faustino, Hélio
A1 López García, Fernando
A1 Mascareñas Cid, José Luis
K1 Alkynes
K1 Azides
K1 Chemical ligations
K1 Click chemistry
K1 Ruthenium
AB The development of efficient metal-promoted bioorthogonal ligations remains as a major scientific challenge. Demonstrated herein is that azides undergo efficient and regioselective room-temperature annulations with thioalkynes in aqueous milieu when treated with catalytic amounts of a suitable ruthenium complex. The reaction is compatible with different biomolecules, and can be carried out in complex aqueous mixtures such as phosphate buffered saline, cell lysates, fetal bovine serum, and even living bacteria (E. coli). Importantly, the reaction is mutually compatible with the classical CuAAC
PB Wiley
YR 2017
FD 2017-08-03
LK http://hdl.handle.net/10347/15804
UL http://hdl.handle.net/10347/15804
LA eng
NO P. Destito, J. R. Couceiro, H. Faustino, F. López, J. L. Mascareñas, Angew. Chem. Int. Ed. 2017, 56, 10766
NO This work has received financial support from Spanish grants(SAF2016-76689-R and SAF2013-41943-R), the Xunta deGalicia (2015-CP082 and Centro Singular de Investigaciln deGalicia accreditation 2016-2019 ED431G/09), the EuropeanUnion (European Regional Development Fund - ERDF),and the ERC (Adv. Grant 340055)
DS Minerva
RD 26 abr 2026