RT Journal Article
T1 Mitral cell development in the olfactory bulb of sharks: evidences of a conserved pattern of glutamatergic neurogenesis
A1 Docampo Seara, Alberto
A1 Lanoizelet, M.
A1 Lagadec, Ronan
A1 Mazan, Sylvie
A1 Candal Suárez, Eva María
A1 Rodríguez Díaz, Miguel Ángel
K1 Olfactory bulb
K1 Catshark
K1 Mitral cells
K1 Development
K1 Glutamatergic lineage
K1 Pax6
AB In mammals, the development of the olfactory bulb (OB) relies in part on the expression of transcription factors involved in the specifications/differentiation of glutamatergic cells. In a previous study from our group, a high molecular similarity was reported between mammals and cartilaginous fishes regarding the neurogenic mechanisms underlying the development of glutamatergic cells in the telencephalon. However, information about the transcriptional program operating in the development of the glutamatergic system (mainly represented by mitral cells) in the OB is lacking in the catshark Scyliorhinus canicula, a cartilaginous fish. Using immunohistochemistry and in situ hybridization techniques, we have found that, previously to the appearance of the olfactory primordium (OP), proliferating cells expressing Pax6 with molecular hallmarks of progenitor radial glia were located in the ventrolateral pallial ventricular zone. Later in development, when the OP is recognizable, a stream of Pax6-positive cells were observed between the ventricular zone and the OP, where transcription factors involved in mitral cell development in mammals (ScTbr2, ScNeuroD, Tbr1) are expressed. Later in development, these transcription factors became expressed in a layered-like structure where ScVglut1, a marker of mitral cells, is also present. Our data suggest that the transcriptional program related with the specification/differentiation of glutamatergic cells in the telencephalon has been conserved throughout the evolution of vertebrates. These results, in combination with previous studies concerning GABAergic neurogenesis in sharks, have evidenced that the OB of mammals and sharks shares similarities in the timing and molecular programs of development.
PB Springer
SN 1863-2653
YR 2019
FD 2019
LK http://hdl.handle.net/10347/21334
UL http://hdl.handle.net/10347/21334
LA eng
NO Docampo-Seara, A., Lanoizelet, M., Lagadec, R. et al. Mitral cell development in the olfactory bulb of sharks: evidences of a conserved pattern of glutamatergic neurogenesis. Brain Struct Funct 224, 2325–2341 (2019). https://doi.org/10.1007/s00429-019-01906-9
NO This work was supported by the Spanish Ministerio de Economía y Competitividad-FEDER (BFU2014-5863-1P and BFU2017-8986-1P) and CNRS Université Pierre et Marie Curie Grant No. ANR-16-CE13-0013-02
DS Minerva
RD 3 may 2026