RT Journal Article
T1 Development and use of an indirect ELISA in an outbreak of bovine besnoitiosis in Spain
A1 Fernández García, Aurora
A1 Álvarez García, Gema
A1 Risco Castillo, Verónica
A1 Aguado Martínez, Adriana
A1 Marcén, J. M.
A1 Rojo Montejo, Silvia
A1 Castillo, J. A.
A1 Ortega Mora, Luis Miguel
K1 Bovine besnoitiosis
K1 Indirect ELISA
AB An indirect ELISA based on a soluble extract of Besnoitia besnoiti tachyzoites was developed and standardised. A set of positive and negative reference bovine sera were characterised using an immunofluorescence antibody test and Western blot. A cut-off with a relative index per cent of 8.1 was determined for equal sensitivity and specificity (100 per cent) by twograph receiver operating characteristic  analysis. Cross-reactions with other closely related Apicomplexan parasites were discarded. The standardised ELISA was then used during an outbreak of bovine besnoitiosis in a mountainous area of central Spain. The outbreak occurred in nine herds, and 358 animals that shared grazing lands during the summer season were affected. Clinical examination and blood sampling were carried out for all animals, and skin biopsies were obtained from animals with skin lesions. The confirmatory diagnosis was carried out by means of the indirect ELISA, together with the identification of tissue cysts by microscopy. Most of the animals were seropositive (90·5 per cent), but only 43 per cent of seropositive cattle developed clinical signs compatible with besnoitiosis. Additionally, a significant increase in seroprevalence and clinical signs was found to be associated with the increasing age of the animals, suggesting rapid horizontal transmission of the disease.
PB Wiley
SN 0042-4900
YR 2010
FD 2010
LK https://hdl.handle.net/10347/39497
UL https://hdl.handle.net/10347/39497
LA eng
NO Fernández-García, A., Álvarez-García, G., Risco-Castillo, V., Aguado-Martínez, A., Marcén, J.M., Rojo-Montejo, S., Castillo, J.A. and Ortega-Mora, L.M. (2010), Development and use of an indirect ELISA in an outbreak of bovine besnoitiosis in Spain. Veterinary Record, 166: 818-822. https://doi.org/10.1136/vr.b4874
NO This is the peer reviewed version of the following article: Fernández-García, A., Álvarez-García, G., Risco-Castillo, V., Aguado-Martínez, A., Marcén, J.M., Rojo-Montejo, S., Castillo, J.A. and Ortega-Mora, L.M. (2010), Development and use of an indirect ELISA in an outbreak of bovine besnoitiosis in Spain. Veterinary Record, 166: 818-822, which has been published in final form at https://doi.org/10.1136/vr.b4874. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
NO The work was partially funded by grant 147/2005 from Comunidad Foral de Navarra.
DS Minerva
RD 28 abr 2026