RT Journal Article
T1 Interlaboratory Evaluation of Multiple LC–MS/MS Methods and a Commercial ELISA Method for Determination of Tetrodotoxin in Oysters and Mussels
A1 Turner, Andrew D.
A1 Dean, Karl J.
A1 Dhanji-Rapkova, Monika
A1 Dall’Ara, Sonia
A1 Pino, Florella
A1 McVey, Claire
A1 Haughey, Simon
A1 Logan, Natasha
A1 Elliot, Christopher
A1 Gago Martínez, Ana
A1 Leao, José Manuel
A1 Giráldez, Jorge
A1 Gibbs, Ryan
A1 Thomas, Krista
A1 Pérez-Calderón, Ruth
A1 Faulkner, Dermot
A1 McEneny, Hugh
A1 Savar, Veronique
A1 Reveillon, Damien
A1 Hess, Philipp
A1 Arévalo, Fabiola
A1 Lamas Castro, Juan Pablo
A1 Cagide, Eva
A1 Álvarez, Mercedes
A1 Antelo Queijo, Álvaro
A1 Klijnstra, Mirjam D.
A1 Oplatowska-Stachowiak, Michalina
A1 Kleintjens, Tim
A1 Sajic, Nermin
A1 Boundy, Michael J.
A1 Maskrey, Benjamin
A1 Harwood, D. Tim
A1 González Jartín, Jesús María
A1 Alfonso Rancaño, María Amparo
A1 Botana López, Luis Miguel
K1 Farmacoloxía veterinaria
K1 Medicamentos veterinarios
K1 Tetrodotoxins
K1 Oysters
K1 Mussels
K1 Espectrometría
AB BackgroundGiven the recent detection of tetrodotoxin (TTX) in bivalve molluscs but the absence of a full collaborative validation study for TTX determination in a large number of shellfish samples, interlaboratory assessment of method performance was required to better understand current capabilities for accurate and reproducible TTX quantitation using chemical and immunoassay methods.ObjectiveThe aim was to conduct an interlaboratory study with multiple laboratories, using results to assess method performance and acceptability of different TTX testing methods.MethodsHomogenous and stable mussel and oyster materials were assessed by participants using a range of published and in-house detection methods to determine mean TTX concentrations. Data were used to calculate recoveries, repeatability, and reproducibility, together with participant acceptability z-scores.ResultsMethod performance characteristics were good, showing excellent sensitivity, recovery, and repeatability. Acceptable reproducibility was evidenced by HorRat values for all LC–MS/MS and ELISA methods being less than the 2.0 limit of acceptability. Method differences between the LC–MS/MS participants did not result in statistically different results. Method performance characteristics compared well with previously published single-laboratory validated methods and no statistical difference was found in results returned by ELISA in comparison with LC–MS/MS.ConclusionThe results from this study demonstrate that current LC–MS/MS methods and ELISA are on the whole capable of sensitive, accurate, and reproducible TTX quantitation in shellfish. Further work is recommended to expand the number of laboratories testing ELISA and to standardize an LC–MS/MS protocol to further improve interlaboratory precision.HighlightsMultiple mass spectrometric methods and a commercial ELISA have been successfully assessed through an interlaboratory study, demonstrating excellent performance.
PB Oxford Academic
YR 2023
FD 2023
LK http://hdl.handle.net/10347/33710
UL http://hdl.handle.net/10347/33710
LA eng
NO Andrew D Turner, Karl J Dean, Monika Dhanji-Rapkova, Sonia Dall’Ara, Florella Pino, Claire McVey, et al. Interlaboratory Evaluation of Multiple LC–MS/MS Methods and a Commercial ELISA Method for Determination of Tetrodotoxin in Oysters and Mussels. J. AOAC Int., 106(2), 2023, 356–369
NO Materials were prepared at and shipped from Cefas, with laboratory work, statistical assessment and report writing funded by Interreg Alertox-Net EAPA-317–2016 (Atlantic Area Program).
DS Minerva
RD 28 abr 2026