RT Journal Article
T1 Improved triamcinolone acetonide-eluting contact lenses based on cyclodextrins and high hydrostatic pressure assisted complexation
A1 Marto Costa, Carolina
A1 Toffoletto, Nadia
A1 Salema-Oom, Madalena
A1 Antunes, Alexandra M. M.
A1 Pinto, Carlos A.
A1 Saraiva, Jorge A.
A1 Silva Herdade, Ana S.
A1 Álvarez Lorenzo, Carmen
A1 Serro, Ana Paula
K1 Triamcinolone acetonide
K1 Cyclodextrin
K1 Drug-eluting contact lenses
K1 Diabetic macular edema
K1 High hydrostatic pressure sterilization
AB Contact lenses (CLs) constitute an advantageous platform for the topical release of corticosteroids due to their prolonged contact with the eye. However, the lipophilic nature of corticosteroids hampers CLs' ability to release therapeutic amounts. Two approaches to improve loading and release of triamcinolone acetonide (TA) from poly(2-hydroxyethyl methacrylate)-based hydrogels were investigated: adding 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) to the monomers solution before polymerization (HEMA/i-CD) and an hydrogels' post-treatment with HP-β-CD (HEMA/p-CD). The effect of HP-β-CD and sterilization by high hydrostatic pressure (HHP) on the hydrogel properties (water content, oxygen and ion permeability, roughness, transmittance, and stiffness) was evaluated. The HEMA/i-CD hydrogels had stronger affinity for TA, sustaining its release for one day. HHP sterilization promoted the formation of cyclodextrin-TA complexes within the hydrogels, improving their drug-loading capacity »60 %. Cytotoxicity and irritability tests confirmed the safety of the therapeutic CLs. TA released from the hydrogels permeated through ocular tissues ex vivo and showed anti-inflammatory activity. Finally, a previously validated mathematical model was used to estimate the ability of the TA-loaded CLs to deliver therapeutic drug concentrations to the posterior part of the eye. Overall, HP-β-CD-containing CLs are promising candidates for the topical ocular application of TA as an alternative delivery system to intraocular injections
PB Elsevier
SN 0144-8617
YR 2024
FD 2024
LK http://hdl.handle.net/10347/33696
UL http://hdl.handle.net/10347/33696
LA eng
NO Carbohydrate Polymers, Volume 331, 2024, 121880
NO Work supported by Fundação para a Ciência e a Tecnologia (FCT) through [doi: 10.54499/UIDB/00100/2020] and [doi: 10.54499/UIDP/00100/2020] (CQE), [doi: 10.54499/UIDB/04585/2020] (CiiEM) and [doi:10.54499/PTDC/CTM-CTM/2353/2021] (SOL project), University of Aveiro and FCT/MCT through [UIDB/50006/2020 and UIDP/50006/2020] (LAQV-REQUIMTE) co-funded by the FEDER within PT2020 Partnership Agreement, and MCIN/AEI/10.13039/501100011033 Spain [PID 2020-113881RB-I00], Xunta de Galicia [ED431C 2020/17], and FEDER. The authors acknowledge SICASAL - Indústria e Comércio de Carnes, S.A. for the kind supply of porcine fresh eyes and to Dr. Nuno Taveira from the University of Lisbon for providing THP-1 human monocytes cell line. ASHLAND kindly provided Cavasol® W7 HP PHARMA (HP-β-CD)
DS Minerva
RD 28 abr 2026