RT Journal Article
T1 ZFTool: A Software for Automatic Quantification of Cancer Cell Mass Evolution in Zebrafish
A1 Carreira Nouche, María José
A1 Vila Blanco, Nicolás
A1 Cabezas Sáinz, Pablo
A1 Sánchez Piñón, Laura
K1 Xenotransplant
K1 Cancer cells
K1 Zebrafish image analysis
K1 In vivo assay
AB Zebrafish (Danio rerio) is a model organism for the study of human cancer.Compared with the murine model, the zebrafish model has several properties ideal for personalizedtherapies. The transparency of the zebrafish embryos and the development of the pigment-deficient”casper“ zebrafish line give the capacity to directly observe cancer formation and progression in theliving animal. Automatic quantification of cellular proliferation in vivo is critical to the developmentof personalized medicine. Methods: A new methodology was defined to automatically quantify thecancer cellular evolution. ZFTool was developed to establish a base threshold that eliminates theembryo autofluorescence, automatically measures the area and intensity of GFP (green-fluorescentprotein) marked cells, and defines a proliferation index. Results: The proliferation index automaticallycomputed on different targets demonstrates the efficiency of ZFTool to provide a good automaticquantification of cancer cell evolution and dissemination. Conclusion: Our results demonstrate thatZFTool is a reliable tool for the automatic quantification of the proliferation index as a measure ofcancer mass evolution in zebrafish, eliminating the influence of its autofluorescence
PB MDPI
SN 2076-3417
YR 2021
FD 2021
LK http://hdl.handle.net/10347/32003
UL http://hdl.handle.net/10347/32003
LA eng
NO Carreira, M.J.; Vila-Blanco, N.; Cabezas-Sainz, P.; Sánchez, L. ZFTool: A Software for Automatic Quantification of Cancer Cell Mass Evolution in Zebrafish. Appl. Sci. 2021, 11, 7721. https://doi.org/ 10.3390/app11167721
NO This work has received financial support from Consellería de Cultura, Educación e OrdenaciónUniversitaria (accreditation 2019-2022 ED431G-2019/04) and the European Regional DevelopmentFund (ERDF), which acknowledges the CiTIUS-Research Center in Intelligent Technologies ofthe University of Santiago de Compostela as a Research Center of the Galician University System
DS Minerva
RD 28 abr 2026