RT Journal Article
T1 Canonical and novel non-canonical activities of the Holliday junction resolvase Yen1
A1 Carreira Rodríguez, Raquel
A1 Aguado Domínguez, Francisco Javier
A1 Hurtado Nieves, Vanesa
A1 González Blanco, Miguel
AB Yen1 and GEN1 are members of the Rad2/XPG family of nucleases that were identified as the first canonical nuclear Holliday junction (HJ) resolvases in budding yeast and humans due to their ability to introduce two symmetric, coordinated incisions on opposite strands of the HJ, yielding nicked DNA products that could be readily ligated. While GEN1 has been extensively characterized in vitro, much less is known about the biochemistry of Yen1. Here, we have performed the first in-depth characterization of purified Yen1. We confirmed that Yen1 resembles GEN1 in many aspects, including range of substrates targeted, position of most incisions they produce or the increase in the first incision rate by assembly of a dimer on a HJ, despite minor differences. However, we demonstrate that Yen1 is endowed with additional nuclease activities, like a nick-specific 5′-3′ exonuclease or HJ arm-chopping that could apparently blur its classification as a canonical HJ resolvase. Despite this, we show that Yen1 fulfils the requirements of a canonical HJ resolvase and hypothesize that its wider array of nuclease activities might contribute to its function in the removal of persistent recombination or replication intermediates
PB Oxford University Press
SN 0305-1048
YR 2022
FD 2022
LK http://hdl.handle.net/10347/28889
UL http://hdl.handle.net/10347/28889
LA eng
NO Nucleic Acids Research, Volume 50, Issue 1, 11 January 2022, Pages 259–280, https://doi.org/10.1093/nar/gkab1225
NO The Blanco lab was supported from MCIN / AEI / 10.13039/501100011033 [PID2020-115472GB-I00]; MCIN / AEI / 10.13039/501100011033 / FEDER ‘Una manera de hacer Europa’ [BFU2016-78121-P]; Xunta de Galicia (XdG) / FEDER ‘Una manera de hacer Europa’ [ED431F-2016/019, ED431B-2016/016 and ED431C 2019/013]; CIMUS receives financial support from the XdG / FEDER [ED431G 2019/02, Centro Singular de Investigación de Galicia, accreditation 2019–2022]; F.J.A. and R.C. were recipients of pre-doctoral fellowships from XdG [ED481A-2015/011 and ED481A-2018/041]; V.H.-N. from MINECO and AEI [BES-2014-068734]. Funding for open access charge: Ministerio de Ciencia e Innovación and Agencia Estatal de Investigación [PID2020-115472GB-I00]
DS Minerva
RD 28 abr 2026